Measurement and purification of Alanine aminotransferase (ALT) enzyme activity in patients with celiac disease

Main Article Content

Taghreed U. Mohammed
Jinan Hussein Murtadha
Iman Hashim Abdul Razzaq

Abstract

Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance. The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease. Alanine aminotransferase (ALT) activity has been assayed in (30) women serum samples with celiac disease, age range between (20-40) year and (30) serum of healthy women as control group, age range between (22-38) year. In the present study, the mean value of ALT activity was significantly higher in patients with celiac disease than healthy group (p<0.01). The ALT enzyme was partial purified from sera women with celiac disease by dialysis, gel filtration using Sephadex G- 50 and ion exchange chromatography using DEAE- cellulose A-50 . The results showed a single peak by using gel filtration and the activity reached 31-15 U/L .Two isoenzymes were obtained by using ion exchange chromatography and the purity degree of isoenzymse (I, II) were (5.7) and (5.53) fold respectively.

Article Details

How to Cite
1.
Measurement and purification of Alanine aminotransferase (ALT) enzyme activity in patients with celiac disease. Baghdad Sci.J [Internet]. 2017 Sep. 3 [cited 2024 Mar. 28];14(3):0557. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2395
Section
article

How to Cite

1.
Measurement and purification of Alanine aminotransferase (ALT) enzyme activity in patients with celiac disease. Baghdad Sci.J [Internet]. 2017 Sep. 3 [cited 2024 Mar. 28];14(3):0557. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2395

Similar Articles

You may also start an advanced similarity search for this article.