Partial Purification and Characterization of Catalase from Banana Peels Layla O. Farhan | Seenaa A. Mustafa | Areej Sh. Hameed

Main Article Content

Baghdad Science Journal

Abstract

Catalase (EC 1.11.1.6) is a well known enzyme which exists in almost all living creatures exposing to oxygen (such as plants, bacteria, and animals). It is a very necessary enzyme to protect the cell from oxidative detriment by reactive oxygen species (ROS). The aim of this study is the partial purification and characterization of Catalase enzyme from Banana peels.
In this study, fresh banana peels are treated with 70 % ethanol ,further separated with chloroform ,water and ethyl acetate respectively .The supernatant of the enzymatic sample which is treated with chloroform is loaded into gel filtration column with Sephadex G-100 (1.0 x 90 cm) equilibrated with pH7 buffer media (phosphate buffer 0.1 M). Kinetic studies of the purified enzyme activity are measured and characterized .The maximal activity (26.04 units/mg) of catalase is observed with chloroform buffer extraction. The kinetics of catalase; Michalis constant Km and maximum velocity Vmax is determined using Linweaver- Burk plot, The Km value for catalase (434.7mM), Vmax (100 m mole min -1). Characterization results demonstrate that the optimal pH for activity is (7.6). And the optimal temperature for activity is 30?C .The present study indicates that Banana peels is a good source of catalase enzyme.

Downloads

Download data is not yet available.

Article Details

How to Cite
1.
Journal BS. Partial Purification and Characterization of Catalase from Banana Peels. BSJ [Internet]. 5Jun.2016 [cited 5Aug.2020];13(2.2NCC):0392. Available from: http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2784
Section
article

Most read articles by the same author(s)

1 2 3 4 5 6 7 8 9 10 > >>