Baghdad Science Journal

New record of three species of Leeches (Annelida:Hirudinea) in Al-Hindyia River / Babil Province / Iraq

2019-11-16T17:30:06+00:00

In this study, a total of 209 individuals of leeches were collected from Al-Hindyia River / Babil Province. 116 individuals were identified as Erpobdella octaculata (Linnaeus, 1758), 50 individuals as Erpobdella punctata (Leidy,1870) and 43 individuals as Hemiclepsis marginata (Müller, 1774). Four samples were collected monthly during a period from February to June 2018. Some physical and chemical water properties were also examined, including air and water temperature, potential of hydrogen pH, Electrical Conductivity EC, Total Dissolved Solid TDS, Dissolved Oxygen DO, and the Biological Oxygen Demand BOD₅. Air and water temperature were ranged 19.5-29, & 14.6-23.2 °C respectively. The values of pH ranged 6.2-7.6. EC ranged 1104-1581 μs/cm². The TDS recorded 669- 767 mg/l, while the DO reached 1.3- 8.5 mg / l, the BOD₅ ranged 3.5- 5.7 mg/l.

Detection of CTX-M-type ESBLs from Escherichia coli Clinical Isolates from a Tertiary Hospital, Malaysia

2019-11-16T17:30:11+00:00

The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. One hundred of E. coli isolates were collected from different clinical samples from a tertiary hospital. ESBL positivity was determined by the disk diffusion method. PCR used for amplification of CTX-M-type ESBL produced by E. coli. Out of 100 E. coli isolates, twenty-four isolates (24%) were ESBL-producers. E. coli isolated from pus was the most frequent clinical specimen that produced ESBL (41.66%) followed by urine (34.21%), respiratory (22.23%), and blood (19.05%). After PCR amplification of these 24 isolates, 10 (41.66%) isolates were found to possess CTX-M genes. The CTX-M type ESBL producing E. coli against antibiotics belonging to different families showed the highest resistance rates to Ampicillin (100%), Cefotaxime (97%), Cefuroxime (95%), and Ciprofoxacin (86%). Carbapenem groups of antibiotics, Meropenem (89%) and Imipenem (85%) have the highest susceptibility rate among all antibiotics used in this study. The outcome of the antimicrobial susceptibility testing of significant CTX-M- type ESBL producing E. coli could be useful to avoid failure or prolong treatments.

Comparative Antimicrobial Activity of Silver Nanoparticles Synthesized by Corynebacterium glutamicum and Plant Extracts

2019-11-16T17:30:11+00:00

Biosynthesis of nanoparticles has received considerable attention due to the growing need to develop environmentally benign nanoparticle synthesis processes that do not use toxic chemicals. Therefore, biosynthetic methods employing both biological agents such as bacteria and fungus or plant extracts have emerged as a simple and a viable alternative to chemical synthetic and physical method .It is well known that many microbes produce an organic material either intracellular or extracellular which is playing important role in the remediation of toxic metals through reduction of metal ions and acting as interesting Nano factories. As a result, in the present study Ag NPs were synthesized by two methods biosynthetic technique using supernatant of Corynebacterium glutamicum that isolated from soil and green synthesis method by using plant extracts of fresh green plants.Ag NPs which synthesized by two methods were investigated visually by monitoring the color shift of reaction mixture from pale yellow to brown color, UV-Visible spectrophotometer was used to measure maximum absorbance of synthesized Ag NPs. The nanoparticles synthesized from Corynebacterium glutamicum exhibited maximum antimicrobial activity against selected pathogenic and environmental strains more than Ag NPs synthesized by green synthesis method from Spinacia oleracea, Malva parviflora and Eruca sativa. plant extracts

Studying the Role of IFN-γ, Vitamin C, SOD, LH, FSH, APA and ACA in Toxoplasma gondii Infected Miscarriage Women

2019-11-16T17:30:11+00:00

This study is designed to detect the level of cytokine IFN-γ concentration, and some antioxidants, including super oxide dismutase (SOD) and Vitamin C, and to estimate the level of sex hormones (FSH and LH), and to determine auto-antibodies (antiphospholipid antibodies (APA) IgG\IgM, and anticardiolipin antibodies (ACA) IgG\IgM) and to estimate the blood parameters in 51 miscarriage women infected with T.gondii distributed depending on the type of antibodies. Additionally, 39 volunteers non-infected with T.gondii included (19 miscarriage women, 10 pregnant women and 10 non-pregnant women). ELISA and spectrophotometer method were used in this study. The results of IFN-γ showed a significant increase)p<0.05) in the level of IFN-γ in women infected with T.gondii compared with volunteer groups. The current study shows a significant difference in the level of hormones, where the concentration of LH hormone increased in married non-pregnant women compared with other groups, while the concentration of FSH hormone increased in toxoplasmosis women compared with volunteer groups. The results of antioxidants showed no significant difference in Vitamin C activity in infected women compared with volunteer groups, while super oxide dismutase (SOD) activity was significantly lower in infected women compared with volunteer groups. The results of autoantibodies showed a significant difference in anticardiolipin antibodies (ACA) IgM level, the highest level was in aborted women infected with acute infection (IgM Ab), while the anticardiolipin antibodies (ACA) IgG level recorded the highest level in aborted women infected with both acute and chronic infection (IgG&IgM)). The results showed no significant difference in antiphospholipid antibodies (APA) IgM level in infected women when compared to the volunteer groups, while the results showed significant difference in antiphospholipid antibodies (APA) IgG level, where the highest concentration was in the volunteer groups (pregnant and non-pregnant married women). The results of blood parameters showed significant differences in differential leucocytes except for Basophils and Monocytes, and the results showed significant differences in differential erythrocytes, HB, PLT, and PCV.

The Antibacterial, Antiheamolytic, and Antioxidant Activities of Laurus nobilis and Alhagi maurorum Native to Iraq.

2019-11-16T17:30:10+00:00

Ethanolic crude extracts of leaves from Laurus nobilis and Alhagi maurorumfor were screened for alkaloids, saponins, tannins, anthraquinones, steroids, flavonoids, glycosides, and glucosides contents. Biochemical activities, including antibacterial activity, antioxidant, and antihemolytic activity, were investigated. Antibacterial activity against Three types of pathogenic bacteria was detected by disc diffusion analysis and characterized by zone of inhibition (ZOI). Antioxidant properties were determined by a diphenyl-1- picrylhydrazyl (DPPH) method. Results revealed that the inhibitory activity of the plants against G+ve and G-ve bacteria were different, where the greatest ZOI of Alhagi maurorum against Staphylococcus aureus was 12.66 mm, while its effect against Pseudomonas aeruginosa generated an 8.33 mm ZOI, with no did not effects on Escherichia coli. Extract of Laurus nobilis against Escherichia coli with a ZOI reached to 10.33 mm, but did not significantly influence the growth of Staphylococcus aureus and Pseudomonas aeruginosa. When the plant extracts were mixed in equal quantities, the percentage of the effect were increased, where the greatest effect of the mixed observed in E. coli was a 16.66mm ZOI, and in Staphylococcus aureus to 15.66mm. In Pseudomonas aeruginosa the ZOI reached to a 12.33 mm. This is referred to as synergistic effect between these plants against pathogenic bacteria. The extracts did not have any toxic effects on human red blood cells.

Antimicrobial Resistance of Aeromonas salmonicida Isolated From Common carp (Cyprinus carpio) Fishes in Erbil City/ Iraq

2019-11-16T17:30:10+00:00

Aeromonas salmonicida is a fish pathogen and recognized to cause a variety of diseases in humans. There are a few information about A.salmonicida in Iraq and there is no any previous molecular study on it. During the period of December 2017 to May 2018; Sixteen isolates of the A. salmonicida were isolated and identified from 300 common carp (Cyprinus carpio) fishes stomach in aquarium of Erbil city/ Iraq by using manual, automated Vitek 2 compact system, and confirmed by PCR using gene TonB-dependent siderophore (364bp). Antimicrobial susceptibility was determined by disk diffusion method and the results found that all isolates 100% susceptible to imipenem, 100% resistant to nalidixic acid and variable resistance to other studied antibiotics. The antibacterial effect of aqueous and alcohol extracts of Eminium spiculatum was studied by MIC and Molecular studies. The results found that aqueous and alcohol extracts of E. spiculatum have inhibitory effect and the MIC were 1400, 1800 µg/ml respectively. The sub MIC was used for both plant extracts, in plasmid profile the number of plasmid DNA was differ when treated with plant extracts. The inhibitory effect of the plant extracts against total proteins was studied by SDS- polyacrylamide gel electrophoresis and the results showed that there were variations in the protein bands in studied isolates and induction of new bands after treating with plant extracts. It was concluded that the leaves extracts of E.spiculatum could be used as antimicrobial for treatment of A. salmonicida infections and the results were confirmed by molecular studies.

Investigating the Histological Changes in Heart, Lung, Liver and Kidney of Male Albino Mice Treated with Ivabradine

2019-11-16T17:30:09+00:00

The present study aimed to investigate the histological changes of heart, lung, liver and kidney which caused by different concentrations (10, 20 and 40 mg/kg) of Ivabradine. Results of the study revealed some histological changes represented by aggregation of the lymphocytes around respiratory bronchioles of the lung. In the liver, the drug caused hepatocyte necrosis and infiltration of the lymphocytes. In Kidney, there are no histopathological modifications in the tissue after the animals treated with 10 mg\kg of Ivabradine. When the animals treated with Ivabradine drug at 20mg/kg of bw, dose showed vascular congestion between myocardial fibers of heart. Emphysematous changes of the alveoli and infiltration of lymphocytes around respiratory bronchioles of lung. In the liver there were dilated blood sinusoids. Also, there are vascular congestion and congestion of capillaries in the glomerular of kidney. Male mice treated with Ivabradine drug at 40 mg/kg of bw cause increase spaces between myocardial fibers, cardiac atrophy and myocardial degeneration in the heart. In addition, there are infiltration of lymphocytes around respiratory bronchioles, pulmonary congestion and emphysematous changes of the alveoli in lung. In the liver, the drug cause amyloid deposition and degeneration of hepatocytes. Furthermore, the drug caused vascular congestion in the kidney. Conclusion: From the current study, we conclude that the different concentrations of Ivabradine caused tissue changes in the heart, lung, liver and kidneys. The study should continue using different drugs and concentrations.

Geometry, and Normal Modes of Vibration (3N-6) for Di and Tetra-Rings Layer (6, 0) Linear (Zigzag) SWCNTs; A DFT Treatment

2019-11-16T17:30:09+00:00

Density Functional Theory (DFT) method of the type (B3LYP) and a Gaussian basis set (6-311G) were applied for calculating the vibration frequencies and absorption intensities for normal coordinates (3N-6) at the equilibrium geometry of the Di and Tetra-rings layer (6, 0) zigzag single wall carbon nanotubes (SWCNTs) by using Gaussian-09 program. Both were found to have the same symmetry of D_6d point group with C--C bond alternation in all tube rings (for axial bonds, which are the vertical C--Ca bonds in rings layer and for circumferential bonds C—Cc in the outer and mid rings bonds). Assignments of the modes of vibration IR active and inactive vibration frequencies (symmetric and asymmetric modes) based on the image modes applied by the Gaussian 09 display. The whole relations for the vibration modes were also done including nCH stretching, nC--C stretching, δCH, δring (δC--C--C) deformation in plane of the molecule) and gCH, gring (gC--C--C) deformation out of plane of the molecule. The assignment also included modes of puckering, breathing and clock-anticlockwise bending vibrations.

Kinetic Studies of Na+/K+-ATPase in Tissue Aerobic Thyroid Patients

2019-11-16T17:30:09+00:00

Na⁺/K⁺-ATPase is a prevalent enzyme that maintains the Na⁺ and K⁺ gradients across the cell membrane by transporting three Na⁺ out and two K⁺ into the cell, the aim of this study is to provide detailed mechanistic insights, potentially with important effects on physiological regulation of active Na and K transport in tissues of Aerobic Thyroid Patient. Thyroid tissues were obtained from a 35 year old patients, the operation was carried out at the Al-Hadi Specialist Hospital in Samarra city, the sample was stored at -20^ºC until used. The purification protocol included Salt Precipitation, Ion Exchange Chromatography, Gel Filtration and Electrophoresis, a spectrophotometric method was used to determine the enzyme activity. kinetic parameters was also obtained for the enzyme. Partial purification of Na⁺/K⁺-ATPase revealed two isoenzymes (I ,II). The purity of separated isoenzymes were proved by SDS-PAGE electrophoresis. The kinetic characteristics of Na⁺/K⁺-ATPase showed that optimum substrate concentration about 1.5mM, K_m 1.052mM, and V_max 6.062, optimum temperature was 37^ºC, optimum pH 7.4 and optimum time in 25 min. Na⁺/K⁺-ATPase purified from Thyroid tissue has distinct kinetic characteristic that reflects the importance of intracellular regulation of specific Na⁺/K⁺-ATPase pump which gives cells the ability to precisely coordinate to their physiological requirements .

A Molecular and Biochemical Study for Cholesteryl Ester Transfer Protein (CETP) Taq1B in Iraqi Patients with Hyperlipidemia

2019-11-16T17:30:08+00:00

Cholesteryl ester transfer protein gene contains some single nucleotide polymorphisms, which have been associated with serum high-density lipoprotein concentration and other lipoproteins. This study is done for determining of cholesteryl ester transfer protein polymorphism and evaluate its effect on serum lipid profile concentrations in some hyperlipidemic patients compared with healthy subjects in Salah Al-din governorate-Iraq. Blood samples were taken from (90) patients suffering from hyperlipidemia, and (70) samples that were apparently healthy controls. Serum lipid concentrations were measured by enzymatic assays. The polymorphism was genotyped using polymerase chain reaction restriction fragment length polymorphism analysis. The results showed that there was a significant decrease (P<0.05) in the frequency B2 allele, and B1B2, B2B2 genotype, and a significant increase (P<0.05) in the frequency B1 allele, and B1B1 genotype between patients and controls groups. There was a non-significant decrease in the levels of high density lipoproteins, total cholesterol, low density lipoproteins, and very low density lipoproteins levels, and non-significant increase in levels of triglycerides in individuals with the B1B1 genotype than in the B1B2 and B2B2 genotype. However, high density lipoproteins showed a significant decrease (P<0.001) between individuals with the B1B1 genotype and B2B2 genotype. Also, there was a non-significant difference in the levels of high density lipoproteins, total cholesterol, low density lipoproteins, and very low density lipoproteins levels, in individuals with the B1B2 genotype when compared with that of the B2B2 genotype.

Synthesis and Characterization of New Selenonitrone Derivative and Its Effect on Breast Cancer Cell Line Viability in Vitro

2019-11-16T17:30:08+00:00

New nitrone and selenonitrone compounds were synthesized. The condensation method between N-(2-hydroxyethyl) hydroxylamine and substituted carbonyl compounds such as [benzil, 4, 4́-dichlorobenzil and 2,2́ -dinitrobenzil] afforded a variety of new nitrone compounds while the condensation between N-benzylhydroxylamine and substituted selenocarbonyl compounds such as [di(4-fluorobenzoyl) diselenide and (4-chlorobenzoyl selenonitrile] obtained selenonitrone compounds. The condensation of N-4-chlorophenylhydroxylamine with dibenzoyl diselenide obtained another type of selenonitrone compounds. The structures of the synthesized compounds were assigned based on spectroscopic data (FT-IR, ¹H-NMR, ¹³C-NMR, MS and elemental analysis. The result spectra and the result of elemental analysis were verified the expected structure. The results of new selenonitron derivative (N₁) effect on the MDA-MB-231 breast cancer cells in vitro revealed that is an increase in the proliferation of cells with increased concentrations of selenonitron at all periods of the times.

Spectral Properties of Hybrid of Rhodamine (6G) Dyes Doped Epoxy Resin Dissolved in Chloroform

2019-11-16T17:30:07+00:00

The research is dealing with the absorption and fluorescence spectra for the hybrid of an Epoxy Resin doped with organic dye Rhodamine (R6G) of different concentrations (5*10^-6, 5*10^-5,1*10^-5, 1*10^-4, 5*10^-4) Mol/ℓ at room temperature. The Quantum efficiency Q_fm, the rate of fluorescence emission K_fm (s^-1), the non-radiative lifetime τ_fm (s), fluorescence lifetime τ_f and the Stokes shift were calculated. Also the energy gap (Eg) for each dye concentration was evaluated. The results showed that the maximum quantum efficiency 62 % and maximum stokes shift 96 nm was obtained in dye concentration 5*10^-6 and 1*10^-4. The energy gap ranges between 1.066 eV to 1.128 eV depending proportionally on the dye concentrations.

Thermal Conductivity Performance of Silica Aerogel after Exposition on Different Heating under Ambient Pressure

2019-11-16T17:30:07+00:00

The varied thermal conductivity (insulation) of silica aerogel with heating for different pH has been investigated, it has been depended on ambient pressure drying method in the preparing silica aerogel samples, also six different pH of samples (1, 2, 3, 7, 8 and 9) were treated under five degree of heating with (50,100,150,200 and 250) ᴼC. This technique is important to test the carry-outs hydrophobic silica to temperature without high-quality material changes in the basic characteristics. The hot-wire technique is used in this work to examine the thermal conductivity, Fourier Transform Infrared Spectroscopy (FTIR) depended to characterize the bonds and their artificial by heating. Results show that the samples affected by heating through decreasing the density leading to obtaining more insulation metal, moreover varied pH is an important role in thermal conductivity. The average thermal conductivity of all aerogel samples in this work is (0.01- 0.0061 mW m^-10C^-1) this means that it is still below thermal conductivity for air equals to (0.02257) mW m^-10C^-1. Meanwhile, we concluded that the insulation property of silica aerogel is affected by heat treatment and gives it more thermal insulation property.

Estimation of Survival Function for Rayleigh Distribution by Ranking function:-

2019-11-16T17:30:07+00:00

In this article, performing and deriving te probability density function for Rayleigh distribution is done by using ordinary least squares estimator method and Rank set estimator method. Then creating interval for scale parameter of Rayleigh distribution. Anew method using is used for fuzzy scale parameter. After that creating the survival and hazard functions for two ranking functions are conducted to show which one is beast.

Representation of Algebraic Integers as Sum of Units over the Real Quadratic Fields

2019-11-16T17:30:06+00:00

In this paper we generalize Jacobsons results by proving that any integer in is a square-free integer), belong to . All units of are generated by the fundamental unit having the forms

our generalization build on using the conditions

This leads us to classify the real quadratic fields into the sets Jacobsons results shows that and Sliwa confirm that and are the only real quadratic fields in .

New Approach for Solving Three Dimensional Space Partial Differential Equation

2019-11-16T17:30:06+00:00

This paper presents a new transform method to solve partial differential equations, for finding suitable accurate solutions in a wider domain. It can be used to solve the problems without resorting to the frequency domain. The new transform is combined with the homotopy perturbation method in order to solve three dimensional second order partial differential equations with initial condition, and the convergence of the solution to the exact form is proved. The implementation of the suggested method demonstrates the usefulness in finding exact solutions. The practical implications show the effectiveness of approach and it is easily implemented in finding exact solutions.

Finally, all algorithms in this paper are implemented in MATLAB version 7.12.

Hazard Rate Estimation Using Varying Kernel Function for Censored Data Type I

2019-11-16T17:30:05+00:00

In this research, several estimators concerning the estimation are introduced. These estimators are closely related to the hazard function by using one of the nonparametric methods namely the kernel function for censored data type with varying bandwidth and kernel boundary. Two types of bandwidth are used: local bandwidth and global bandwidth. Moreover, four types of boundary kernel are used namely: Rectangle, Epanechnikov, Biquadratic and Triquadratic and the proposed function was employed with all kernel functions. Two different simulation techniques are also used for two experiments to compare these estimators. In most of the cases, the results have proved that the local bandwidth is the best for all the types of the kernel boundary functions and suggested that the 2xRectangle and 2xEpanechnikov methods reflect the best results if compared to the other estimators.

A Semi-Supervised Machine Learning Approach Using K-Means Algorithm to Prevent Burst Header Packet Flooding Attack in Optical Burst Switching Network

2019-11-16T17:30:05+00:00

Optical burst switching (OBS) network is a new generation optical communication technology. In an OBS network, an edge node first sends a control packet, called burst header packet (BHP) which reserves the necessary resources for the upcoming data burst (DB). Once the reservation is complete, the DB starts travelling to its destination through the reserved path. A notable attack on OBS network is BHP flooding attack where an edge node sends BHPs to reserve resources, but never actually sends the associated DB. As a result the reserved resources are wasted and when this happen in sufficiently large scale, a denial of service (DoS) may take place. In this study, we propose a semi-supervised machine learning approach using k-means algorithm, to detect malicious nodes in an OBS network. The proposed semi-supervised model was trained and validated with small amount data from a selected dataset. Experiments show that the model can classify the nodes into either behaving or not-behaving classes with 90% accuracy when trained with just 20% of data. When the nodes are classified into behaving, not-behaving and potentially not-behaving classes, the model shows 65.15% and 71.84% accuracy if trained with 20% and 30% of data respectively. Comparison with some notable works revealed that the proposed model outperforms them in many respects.