New Method for Estimation Mebeverine Hydrochloride Drugs Preparation by a New Analyser: Ayah 6S.X1(WSLEDs)-T.- Two Solar Cells Complied with C.F.I.A

A sensitivity-turbidimetric method at (0-180 o ) was used for det n . of mebeverine in drugs by two solar cell and six source with C.F.I.A.. The method was based on the formation of ion pair for the pinkish banana color precipitate by the reaction of Mebeverine hydrochloride with Phosphotungstic acid. Turbidity was measured via the reflection of incident light that collides on the surface particles of precipitated at 0180 o . All variables were optimized. The linearity ranged of Mebeverine hydrochloride was 0.05-12.5mmol.L 1 , the L.D. (S/N= 3)(3SB) was 521.92 ng/sample depending on dilution for the minimum concentration , with correlation coefficient r = 0.9966while was R.S.D% < 1% of 2,6 mmol.L -1 conc. of Mebeverine hydrochloride. The method is used successfully for three different of target drugs in three different pharmaceutical formulations. A comparison using t-test was studied. It was shown that there is no significant difference between two values.


Figure 1.Chemical structure of Mebeverine hydrochloride
Turbidimetry is defined as the measurement of the degree of attenuation of a radiant beam incident on suspended particles in solution. Nephelometry is the scattered measurement of the light by suspended particles, usually carried out at 90 o (2). The earlier turbidimetry report using the FIA technique for estimating of SO 4 = by monitoring the BaSO 4 suspended particles. Although the use of FIA system with coupled with turbidimetric detection for the determination of different inorganic species in water and plants (3),limited applications to pharmaceutical products were reported (4)(5)(6). Several analytical methods have been developed for the determination of Mebeverine Hydrochloride in lecturer review, Mebeverine Hydrochloride was determined by high performance liquid chromatography (HPLC) (7)(8)(9)(10) , Spectrophotometric (11)(12)(13)(14) .
This research used C.F.I.A with turbidimetric measurement for MVH determination using Ayah (6 source of W.S. LED the two solar cells) analyser. The measurements depends on (pinkish banana color) formation of precipitate particulate for M.V.H-P.T.A. system.

Martial and methods; Chemicals
A standard solution (0.05 mol./L) of Mebeverine hydrochloride (466 g/mol.)was prepared by 5.825g/250 mL. 0.1 mol/L. of Phosphotungstic acid H 3 PW 12 O 40 2880.2 g/mol. Hopkin & Willias) was prepared by 28.802 g /100 mL (a few drops of phosphoric acid were used for dissolving an amount of PTA, and then the solution was heated for completing the dissolution. Followed by completing the required volume and also kept overnight for use). One

Sample Preparation
Twenty tablets were weighted and ground. Each contains 135 mg of Mebeverine hydrochloride which weights (1.46412, 2. 07435, 1.92595) gm corresponding to 699 mg of active ingredient 15 mmol/L for Mebeverine hydrochloride (Mevir-Micro Labs, Meva-Jamjoom pharma, Duspalina-Asia) respectively. This powder was dissolved in D.W. and filtered to remove residue that might affect the measurement. The volume was completed with D.W to 100 mL.

Apparatus
Two channels -Peristaltic pump (GILSON, France) and 6-port injection were used for sample. The measurement was carried out by Ayah 6S.X1-T.-2D. Solar-C.F.I. analyser, depends on flow cell at 2mm path length. The collecting signals were detected by two solar cell (25 mm length X 14 mm width X 1.2 thickness). In addition of x-t. potentiometric recorder for the readout, or digital A.V.O-meter . Turbidometric readings of batch were made by HANNA company. The diagram is shown in Fig. 2 (15) for MVH

Methodology
FIA system for the reaction of MVH-PTA form pinkish banana color precipitate consists of two lines (Fig. 2). The carrier stream (1 line) was the D.W. at 1.8 mL/min that leads to the injection valve to carry Mebeverine hydrochloride as a sample, 140μl ; The second line is PTA solution (7.5 mmol/L) at 1.8 ml/min. Two lines combined at Y-junction, and then the reactant passes through a homemade for measurements (Ayah 6.S. X 1.T.2 D. as a solar cell). Each solution injected was assayed 3 times. The measurements of turbidity at 0-180 o . Mechanism for system MVH-PTA in D.W. in is shown in scheme 1. Using single variable optimization for the conduction of the measurements of MVH-PTA system. Two step study was used.  Table 1 in addition to Fig. 3.A.B. 7.5 mmol/L of PTA was the best for high response. More than 7.5 mmol/L causing accumulation of e particles in the flow cell and leading to restrict the flow, followed by increase of ∆t B of response.   Table 2 and in Fig. 4.A.B. It was found that H 2 SO 4 was the best as a carrier stream for maxima reflection of incident light compared with other used acids and distilled water. Probably, the present of H 2 SO 4 might work as a catalyst for ion pair formation that attributed to the increase in the amount of precipitate.

The Effect of H 2 SO 4 Concentration
The ion pair of MVH (10 mmol/L)-PTA (7.5 mmol/L) system was studied. A solutions H 2 SO 4 (20-60 mmol/L) was prepared. The results obtained in Table 3 Table 4. At slow flow rates, it was noticed that ∆t B of the response increased and the deformation of top maximum peak responses. This might be attributed to the dilution and dispersion factor. While at high flow rate i.e,> 1.8 mL.min -1 , it was noticed that there was a slight increase in peak height, this probably could    Table 5 tabulates all the results for the variation of injected sample volume. An increase in the sample volume led to a significant increase in sensitivity perceptible than low volumes as shown in Fig. 6 A , which shows that the optimum sample volume was 140 μl which gave a regular response

Purge Time
Using the optimum parameters that were achieved in previous section. A study was carried out to establish the optimum allowed permissible time for the sample segment to be injected from the injection valve; 3,5,7,9,7,9,11 and 13 sec. were used for this in addition to allowed the injection valve, Fig 7 A,B and Table 6 show the optimum purge time is 11 sec.

Intensity of Light
By keeping all optimum variables fixed, the variation of light intensity on the efficiency for determination of MVH at 10 mMol/L was optimized by using AVO-meter. Variable intensity of light source was used (0.57-2.29) volt by variation of light intensity knob ( in the front Ayah 6.S.X1-2.D. solar C.F.I. Analyser Fig. 1). The results were tabulated in Table 7 and Fig. 8 with optimum intensity (2.29 volt).

Limit of Detection
The limit of detection was studied at three methods as summed up in Table 9.

Repeat Ability
It is a measurement of precision achieved by the whole assay process. Table 10 sums up measurement of two concentration of MVH each repeated for six successive measurements. Results show that the value of %RSD is less than 1 %   Table 11.  There was no significant difference between the means of Quoted value and the analysed by Ayah 6.S.X1-T.-2.D. for the product of MVH form Saudi-Arabia.
While for the Syria manufacture, a value to beat is 4.303, a calculated t value = 0.213.On the basis the MVH drug produced in Syria if it was compared with the analysis conducted by Ayah 6SX1-T.-2D. and shows no significant difference between the mean of the both Quoted and the one analysed by Ayah 6.S.X1.-T.-2D. Generally, it can be said that there were no significant differences between the three selected available drugs in the market and the analysis method carried out by Ayah 6.S.X1-T.-2.D. Therefore; the analysis carried out by Ayah 6S.X1.-T.-2.D. can be used as an alternative analytical method for whatever these three manufacture were to analyse their product. μ: Quoted value, σ n-1 : Standard deviation, t critical = t tab = t α/2,n-1 = t 0.025,2 =4.303 , n= Three successive measurement (n=3)

Conclusion:
Simple sensitivity application of the proposed of methods to the analysis of Mebeverine hydrochloride in drugs .The method was based on the formation of ion pair for the pinkish banana color precipitate by the reaction of Mebeverine hydrochloride with Phosphotungstic acid. It was shown that there is a good result when compared with Quoted value. An alternative analytical method was found in this research work.