Simultaneous Ratio Derivative Spectrophotometric Determination of Paracetamol, Caffeine and Ibuprofen in Their Ternary Form


  • Khalaf F. Alsamarrai Department of Chemistry, College of Education, University of Samarra, Samarra, Iraq
  • Suham Tawfeq Ameen Department of Medical Laboratory Techiniques, College of Medical & Health Technology Uruk University, Baghdad, Iraq



Caffeine, Ibuprofen, Paracetamol, Peak area, Ratio derivative


A new, accurate, precise and economic two spectrophotometric methods for determination of Paracetamol (Par), Ibuprofen (Ibu), and Caffeine (Caf) were suggested. Those methods were the first and second ratio derivative spectrum using a double devisor. Par, Ibu, and Caf showed many useful peaks for their quantified determination. The validity of all analysis modes for determination of the three compounds, peak to baseline, peak area and peak to peak were according to ICH. The linearity of two methods was between 5 µg/ml as a lower concentration and 50 µg/ml as the highest concentration for three compounds. Recovery percentage was around 100% and relative standard deviation was less than 2.6%. The methods were applied successfully in the determination of Par, Ibu, and Caf in pure and pharmaceutical forms.


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