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Phytochemical profile, Antioxidant, Enzyme inhibitory and acute toxicity activity of Astragalus bruguieri


  • Ahmed Aj Jabbar Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, 44001, Erbil, Iraq
  • Kamaran Kaiani Abdulrahman Chemistry Department, College of Science, Salahaddin University, 44001, Erbil, Iraq
  • Parween Abdulsamad Chemistry Department, College of Education, Salahaddin University, 44001, Erbil, Iraq
  • Sharoukh Mojarrad Biology Department, College of Education, Salahaddin University, 44001, Erbil, Iraq
  • Güldal Mehmetçik Department of Medical biochemistry, Faculty of Medicine, Cyprus International University, Mersin 10, Turkey
  • Abdullah Sh Sardar Biology Department, College of Education, Salahaddin University, 44001, Erbil, Iraq



Acute toxicity, Antioxidant, Astragalus bruguieri, Enzyme inhibitory, Phytochemistry


The medicinal plants (Astragalus species) have been used traditionally as anti-inflammatory, antioxidant, and Anti-diabetics. The current research investigates the phytochemistry and some biological activity of methanol extract of different parts of Astragalus bruguieri Bioss., a wild medicinal plant grows on Safeen mountain, Erbil, Iraq. The methanol extracts of A. bruguieri were analyzed for total phenolic, flavonoid, and saponin contents. In-vitro antioxidant activity was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Furthermore, the plant extracts were examined for in-vitro enzyme inhibitory activity and in-vivo sub-acute toxicity. The results have shown the highest total phenolic (28.83, 20.62 mg GAEs/g extracts) contents, in the leave and root extracts, respectively. While the highest total Flavonoid (50.08, 44.01 mg REs/g) contents, were found in the extracts of aerial parts and leaves, respectively. The total saponin was higher (25.33, 23.18 mg GAEs/g extracts) in the roots and aerial parts, respectively. In-vitro antioxidant   measurement by (DPPH) assay showed leaves as superior part in this activity (42.19mg TEs/g extract), while antioxidant evaluation by (ABTS) assay indicated roots as the most active part (86.90mg TEs/g extract). The α-glucosidase and α-amylase inhibitory activity were found as 0.45-0.67, and 1.2-1.8 mmol ACAEs/g, respectively. The oral acute toxicity test indicated the safety of 600mg/kg dosage of different parts of A. bruguieri on albino rats without behavioral abnormality or mortality. The current study is considered as the first report on the A. bruguieri as a possible new source of biocompatible material for many industrial products.


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