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Indirect Flow Injection Spectrophotometric and Chromatographic Methods for the Determination of Mebendazole in Pharmaceutical Formulations




mebendazole, spectrophotometry, FIA, HPLC, Pharmaceutical products.


Chromatographic and spectrophotometric methods for the estimation of mebendazole in
pharmaceutical products were developed. The flow injection method was based on the oxidation of
mebendazole by a known excess of sodium hypochlorite at pH=9.5. The excess sodium hypochlorite is then
reacted with chloranilic acid (CAA) to bleach out its color. The absorbance of the excess CAA was recorded
at 530 nm. The method is fast, simple, selective, and sensitive. The chromatographic method was carried out
on a Varian C18 column. The mobile phase was a mixture of acetonitrile (ACN), methanol (MeOH), water
and triethylamine (TEA), (56% ACN, 20% MeOH, 23.5% H2O, 0.5% TEA, v/v), adjusted to pH = 3.0 with
1.0 M hydrochloric acid. Naphazoline nitrate was used as an internal standard. The absorption of mebendazole
was measured using a variable wavelength UV detector at 290 nm. Linearity was obtained in the concentration
range of 1-60 and 0.10-3.0 mg/L for the HPLC and FIA, respectively. The methods were applied successfully
for the assay of mebendazole in pharmaceutical products and no interferences were observed from the common
excipients usually used. The proposed methods were validated for their accuracy and precision.


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US Pharmacopoeia 23 NF, Official monographs, Second supplement (1997).

Indian Pharmacopoeia, Ministry of health and family welfare, Government of India, New Delhi (1985).

Argekar AP, Raj SV, Kapadia SU. Simultaneous determination of mebendazole and pyrantel pamoate from tablets by high performance liquid chromatography-reverse phase (RP-HPLC). Talanta 1997; 44(11): 1959-1965. DOI: 10.1016/s0039-9140(96)02118-2.

Poturcu K, Demiralay EC. Determination of Some Physicochemical Properties of Mebendazole with RPLC Method. J. Chem. Eng. Data 2019; 64(6): 2736–2741 (

Parakh DR, Madagul JK, Mene HR, Patil MP, Kshirsagar S. RP-HPLC method development and validation for Quantification of Mebendazole in API and Pharmaceutical Formulations. J. Pharma Tutor. 2016; 4(5): 46-51.

Abbas SM, Jamur JMS, Sallal TD. Indirect Spectrophotometric Determination of Mebendazole Using N-Bromosuccinimide as An Oxidant and Tartarazine Dye as Analytical Reagent. Egypt. J. Chem. 2021; 64(9): 4913-4917 (DOI: 10.21608/EJCHEM.2021.68614.3509).

Dhandar AG, Ganorkar SB, Patil AS, Shirkhedkar AA. Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Quinfamide and Mebendazole in in-house Pharmaceutical Formulation. Journal of Pharmaceutical Technology Research and Management 2018; 6(1): 9-20 (

Swamy N, Basavaiah K. Selective and sensitive assay of mebendazole in pharmaceuticals using bromocresol green by spectrophotometry. Thai Journal of Pharmaceutical Sciences 2013; 37(4): 171-185.

Parakh DR, Patil MP, Sonawane SS, Jain CP. Development and Validation of Spectrophotometric Method for Estimation of Mebendazole in Bulk and Pharmaceutical Formulation. World Journal of Pharmaceutical Research 2015; 4: 2223-2235 ( URL :

Swamy N, Prashanth KN, Basavaiah K. Spectrophotometric Assay of Mebendazole in Dosage Forms Using Sodium Hypochlorite. J Appl. Spectrosc. 2014; 81(3): 525–533 (

Alghanmi RM, Alhazmi LY. Spectrophotometric Determination of Mebendazole Through Charge Transfer Interactions. International Journal of Pharmaceutical Sciences and Research 2019; 10(5): 2504-2515 (DOI: 10.13040/IJPSR.0975-8232.10(5).2504-15).

Lee JS, Cho SH, Lim CM, Chang MI, Joo HJ, Bae H. et al. A Liquid Chromatography – Tandem Mass Spectrometry Approach for the Identification of Mebendazole Residue in Pork, Chicken, and Horse. PLoS ONE 2017; 12(1):

Roshdy A, Elmansi H, Shalan S, Elbrashy A. Use of eosin for green spectroscopic determination of mebendazole. Luminescence 2020; 1-9. (

Al-Momani IF. Spectrophotometric Determination of Selected Cephalosporins in Drug Formulations Using Flow Injection Analysis. J. of Pharmaceutical and Biomedical Research 2001; 25: 751- 757 (DOI: 10.1016/s0731-7085(01)00368-5).

Al-Momani IF, Rababah HM. Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations. International Journal of Pharmacy and Chemistry 2017; 3(4): 52-55 (doi: 10.11648/j.ijpc.20170304.11).

Al-Momani IF, Thalji MR. Indirect Flow-Injection Spectrophotometric Determination pf Some Beta-Lactam Antibiotics. Jordan Journal of Pharmaceutical Sciences 2021; 14(2): 127-136.

Abdulrahman K, Al-Abachi AM, Al-Qaissy MH. Flow injection- Spectrophotometric determination of some catecholamine drugs. Baghdad Sci. J. 2021; 2(1): 124-30.

Shakir IMA, Khudhair BA. A novel online coupling of ion selective electrode with the flow injection system for the determination of vitamin B1. Baghdad Sci. J. 2016; 3.2. 2NCC.