Ghrelin and Obestatin Levels as a Novel Marker in Iraqi Obese Children
Keywords:Ghrelin, Insulin resistance, Lipid profile, Obesity, Obestatin
Obesity is an escalating health problem in developing countries. One to ten children worldwide are overweight in a report showed by the International Obesity Task Force. Ghrelin, orexigenic peptide, has 28 amino acids, it is considered the greatest remarkable promotion in the last two decades for understanding the physiological changes of action regulating food intake and hunger. Obestatin is a 23-amino acid peptide nearly connected to ghrelin that secures from substitution splicing the alike resort molecule, pre-proghrelin. The study is designed to assess the level of ghrelin and obestatin in Iraqi obese children and its relation with other biochemical parameters. Ghrelin and obeststin levels were measured in sixty obese children; the range of their age was (5-16) years, and this group was compared to 30 children in a healthy state as a control. Serum FBS, PPBS, and also lipid profiles (TG, TC, HDL-c, and LDL-c) were determined in the studied groups. Calculation of insulin resistance was done by using (HOMA-IR) a Homeostasis Model assessment of insulin resistance. Ghrelin levels were lower in a significant way in subjects who have obesity in comparison with control group. Significantly, there was a high increase in obestatin in obese patients when compared with control. This study demonstrates that obestatin raises and ghrelin is low in obesity, these hormones are related to the resistance of insulin, and there is an association between ghrelin and oxidative stress. The scales between obestatin and ghrelin are likely disturbed in obesity.
Published Online First 20/2/2023
Abu Baker N N, Daradkeh S M. Prevalence of overweight and obesity among adolescents in Irbid governorate, Jordan. East Mediator Health J. 2010;16.(6):657 662. https://apps.who.int/iris/handle/10665/117935
ALNohair S. Obesity in Gulf Countries. Int J Health Sci. Qassim University. 2014; 8(2): 174-177. https://doi.org/10.12816/0006074
Khan A, Choudhury N, Uddin S, Hossain L, La Baur: Longitudinal trends in global obesity research and collaboration: a review using bibliometric metadata. Obes Rev. 2016; 17: 377–385. https://doi.org/10.1159/00045290.
Sulamin SJ, AL-Ani HM. Prevelance of Obesity and physical activity among Primary school children in Erbil/. Mosul J Nurs. 2020; 8(1): 1-13. https://doi.org/10.33899/mjn.2020.164353
Israa N Z, Harith S. Anemia and The Level of IL-6 in Children Infected with Entamoeba histolytica and Giardia lamblia. Baghdad Sci J. 2021; 18(4) Supplement: 1485-1491. https://doi.org/10.21123/bsj.2021.18.4(Suppl.).1485
Chinar H S, Ridha H H, Ismail M M Ghrelin and Leptin and Their Relations with Insulin Resistance in Diabetes Mellitus Type 2 Patients, Baghdad Sci J. 2022; 19(1): 33 42. https://doi.org/10.21123/bsj.2022.19.1.0033
Cowan E, Burch K J, Green BD, Grieve DJ. Obestatin as a key regulator of metabolism and cardiovascular function with emerging therapeutic potential for diabetes. Br J Pharmecol. 2016;173: 2165–2181. https://doi.org/10.1111/bph.13502.
Ku JM, Andrews ZB, Barsby T, Reichenbach A, Lemus MB, Drummond GR, et al: Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s). Endocrinol¬ogy. 2015; 156: 280–290. https://doi.org/10.1210/en.2014-1415
Rovella V, Rodia G, Di Daniele F, Cardillo C, Campia U, Noce A, et al. Association of Gut Hormones and Microbiota with Vascular Dysfunction in Obesity. Nutrients. 2021; 13(2): 613. https://doi.org/10.3390/nu13020613.
Mariana C T, Eniko C B, Gizella T F, Eniko N N . Involvement of inflammatory cytokines in obesity and its complications. Rev Română de Medicină de Lab. 2018; 26(3): 356-372. https://doi.org/10.2478/rrlm-2018-0019
Barlow SE. Expert committee recommendations regarding the prevention, assessment, and treatment of child and adolescent overweight and obesity: summary report. Pediatrics. 2007; 120(4): S164-92. https://doi.org/10.1542/peds.2007-2329C.
Paul D, Jan W, Jaap S. Body mass index as a measure of body fatness: Age- and sex-specific prediction formulas. Brit J Nut. 2007; 65(2): 105-14. https://doi.org/10.1079BJN199910073.
Judzewitsch R G, Pfeifer M A, Best JD, Beard JC, Halter J B. and Porte D.J. (1982): Chronic chlorpropamide therapy of non insulin-dependent diabetes augments basal and stimulated insulin secretion by increasing islet sensitivity to glucose. J Clin End Metab. 55(2): 321-328. https://doi.org/10.1210/jcem-55-2-321.
Wallace T M, Levy JC, Matthews DR. Use and abuse of HOMA modeling. Diabetes Care. 2004; 27(6): 1487-1495. https://doi.org/10.2337/diacare.27.6.1487
Shams N. Market Analysis of Childhood Obesity 2020. J Child Obes.2019; 4(3): 371: 1–3.
Meyer J F, Larsen S B, Blond K, Damsgaard C T, Bjerregaard L G, Baker J L. Associations between body mass index and height during childhood and adolescence and the risk of coronary heart disease in adulthood: a systematic review and meta‐analysis. Obes Rev. 2021; 22(9): e13276. https://doi.org/10.1111/obr.13276
Bitew Z W, Alemu A, Ayele E G, Tenaw Z, Alebel A, Worku T. Metabolic syndrome among children and adolescents in low and middle income countries: a systematic review and meta-analysis. Diabetol Metab Syndr.202; 12(1); 1-23. https://doi.org/10.1186/s1398-020-00601-8.
Wang Y, Guo S, Zhuang Y, Yun Y, Xu P, He X, Jiang Y. Molecular recognition of an acyl-peptide hormone and activation of ghrelin receptor. Nat comm. 2021; 12(1): 1-9. https://doi.org/10.1038/s41467-02025364-2.
Jurkovičová, J , Hirošová K , Vondrová, D , Samohýl M , Štefániková Z, Filová A, Kachútová I, Babjaková J, Argalášová Ľ. The Prevalence of Insulin Resistance and the Associated Risk Factors in a Sample of 14-18-Year-Old Slovak Adolescents. Inte J env res and pub health. 2021. 18(3): 909. https://doi.org/10.3390/ijerph18030909.
Kansra, Alvina R, Sinduja Lakkunarajah, M, Susan J. Childhood and adolescent obesity: a review. Front Pediatr . 2021;8: 581461. https://doi.org/10.3389/fped.2020.581461.
Gupta D, Ogden S B, Shankar K, Varshney S, Zigman J M. A LEAP 2 conclusions? Targeting the ghrelin system to treat obesity and diabetes. Mol Meta 2021; 46: 101128. https://doi.org/10.1016/j.molmet.2020.101128
Hajishizari S, Imani H, Mehranfar S, Saeed Yekaninejad M, Mirzababaei A, Clark C C, Mirzaei K. The association of appetite and hormones (leptin, ghrelin, and Insulin) with resting metabolic rate in overweight/obese women: a case–control study. BMC Nutr. 2021; 8(1): 1-12. https://doi.org/10.1186/s400795-022-00531.w.
Velez E J, Unniappan S. A comparative update on the neuroendocrine regulation of growth hormone in vertebrates. Front Endocrinol . 2021; 11: 614981. https://doi.org/10.3389/fendo.2020.614981
Razzaghy-Azar M, Nourbakhsh M, Pourmoteabed A, Nourbakhsh M, Ilbeigi D, Khosravi M. An Evaluation of Acylated Ghrelin and Obestatin Levels in Childhood Obesity and Their Association with Insulin Resistance, Metabolic Syndrome, and Oxidative Stress. J Clin Med. 2016 ; 5(7): 61. https://doi.org/10.3390/jcm5070061.
Khwanchuea R. Punsawad C. Associations Between Body Composition, Leptin, and Vitamin D Varied by the Body Fat Percentage in Adolescents. Fron in Endo. 2022; 8(13): 581461. https://doi.org/10.3389/fendo.2022.876231.
Yu A P, Ugwu F N, Tam B T, Lee P H, Lai C W, Wong C S, et al. Ghrelin axis reveals the interacting influence of central obesity and hypertension. Front Endocrinol . 2018; 9: 534. https://doi.org/10.3389/fendo.2018.00534
Sadiq C H, Hussein R H, Maulood I M . Ghrelin and Leptin and Their Relations with Insulin Resistance in Diabetes Mellitus Type 2 Patients. Baghdad Sci J . 2022; 19(1): 0033-0033. https://doi.org/10.21123/bsj.2022.19.1.0033
Sovetkina A, Nadir R, Fung J, Nadjarpour A, Beddoe B. The Physiological Role of Ghrelin in the Regulation of Energy and Glucose Homeostasis. Cureus. 2020; 12(5): e7941. https://doi.org/10.7759/cureus.7941.
Schalla M A, Stengel A. The Role of the Gastric Hormones Ghrelin and Nesfatin-1 in Reproduction. Int J Mol Sci. 22(20), 11059. https://doi.org/10.3390/ijms222011059
Villarreal D, Pradhan G, Zhou Y, Xue B, Sun Y. Diverse and Complementary Effects of Ghrelin and Obestatin. Biomolecules. 2022; 12(4): 517. https://doi.org/10.3390/biom12040517
Ali Ahmad M, Karavetian M, Moubareck C A, Wazz, G, Mahdy T, Venema K . The Association between Peptide Hormones with Obesity and Insulin Resistance Markers in Lean and Obese Individuals in the United Arab Emirates. Nutrients. 2022; 14(6): 1271. https://doi.org/10.3390/nu14061271.
Szentpéteri A, Lőrincz H, Somodi S, Varga V E, Paragh, G, Seres, I, et al. Serum obestatin level strongly correlates with lipoprotein subfractions in non-diabetic obese patients. Lipids Health Dis. 2018; 17(1): 1-8. https://doi.org/10.1186/s12944-014-0691-y
Shankar K, Gupta D, Mani B K, Findley B G, Osborne-Lawrence S, Metzger N P, Zigman J. M. Ghrelin protects against insulin-induced hypoglycemia in a mouse model of type 1 diabetes mellitus. Front Endocrinol. 2020; 11: 606. https://doi.org/10.3389/fendo.2020.00606
Ikenoya C, Takemi S, Kaminoda A, Aizawa S, Ojima S, Gong Z, Sakata I. β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification. Sci Rep. 2018; 8(1): 1-9. https://doi.org/10.1038/s41598-018-27458-2.
Coskun Z M, Sacan O, Karatug A, Turk N, Yanardag, R, Bolkent S , Bolkent S. Regulation of oxidative stress and somatostatin, cholecystokinin, apelin gene expressions by ghrelin in stomach of newborn diabetic rats. Acta Histochem. 2013; 115(7): 740–747. https://doi.org/10.1016/j.acthis.2013.03.001
Aly GS, Hassan NE, Anwar GM, Ahmed HH, El-Masry SA, El-Banna RA, Ahmed NH, Kamal AN, Tarkan RS. Ghrelin, obestatin and the ghrelin/obestatin ratio as potential mediators for food intake among obese children: a case control study. J Pediatr Endocrinol Metab. 2020; 25; 33(2): 199-204. https://doi.org/10.1515/jpem-2019-0286. PMID: 31926094.
Pradhan G, Wu C S, Han Lee J, Kanikarl P, Guo S, Yechoor V K, Sun Y. Obestatin stimulates glucose-induced insulin secretion through ghrelin receptor GHS-R. Sci Rep. 2017; 7(1): 1-10. https://doi.org/10.1038/s41598-01700888-0.
Ouerghi N, Feki M, Bragazzi N L, Knechtle B, Hill L, Nikolaidis P T, etal . Ghrelin Response to Acute and Chronic Exercise: Insights and Implications from a Systematic Review of the Literature. Sports Med. 2021;51(11): 2389-2410. https://doi.org/10.1007/s40279-021-01518-6
Reinehr T, de Sousa G, Roth, C L. Obestatin and ghrelin levels in obese children and adolescents before and after reduction of overweight. Clin Endocrinol. 2008; 68(2): 304–310. https://doi.org/10.1111/j.1365-2265.2007.03042.x.
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