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Isolation and Classification of Green Alga Stigeoclonium attenuatum and Evaluation of its Ability to Prepare Zinc Oxide Nanoflakes for Methylene Blue Photodegradation by Sunlight

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DOI:

https://doi.org/10.21123/bsj.2023.7231

Keywords:

Antibacterial activity, Active biochemical compounds, Nanoparticles, Photocatalyst,: Stigeoclonium attenuatum

Abstract

           Algae have been used in different applications in various fields such as the pharmaceutical industry, environmental treatments, and biotechnology. Studies show that the preparation of nanoparticles by a green synthesis method is a promising solution to many medical and environmental issues. In the current study, the green alga Stigeoclonium attenuatum (Hazen) F.S. Collins 1909 was isolated and identified from the Al-Hillah River (Governorate of Babylon) in the middle of Iraq. The green synthesis by the aqueous extract of algae was used to prepare the nanoflakes of ZnO. Nanoflakes of ZnO are characterized by X-Ray diffraction (XRD) and scanning electron microscope (SEM) with flakes shape and dimensions ranging between 200-500 nm and thickness between 20-23 nm. And this study comprises a test of ZnO nanoflakes efficiency as a photocatalyst factor, thus experiments set of an aqueous solution of methylene blue with ZnO nanoflakes and exposed to sunlight have been conducted. The absorbance of methylene blue at 660 nm reduces over time and almost vanishes between 60-120 minutes. Consequently, it is obvious that in the presence of sunlight, pristine ZnO nanoflakes are photocatalytically active with a degradation efficiency of 97%. Furthermore, the antibacterial activity of ZnO nanoflakes that were prepared by aqueous extract of algae was evaluated against some resistant strains of bacteria Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus sp. and the antibacterial activity of NPs rises as concentration increases 50, 100, and 150 μg/ml.

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References

Prescott GW. Algae of Western great Lakes area.Cranbrook Institute of science bulletin; 1951. p.115

Abuzer C, Hilal B. Biochemical and morphological responses to cadmium-induced oxidative stress in Cladophora glomerata. Turk J Bot.2020 April; 44(3):222-231. https://dx.doi.org/10.3906/bot-2001-12

Shah SA, Hassan SS , Bungau S, Si Y, Xu H, Rahman MH, et al. Chemically Diverse and Biologically Active Secondary Metabolites from Marine Phylum chlorophyte. Mar. Drugs. 2020 Sep; 18(10): 493. https://doi.org/10.3390/md18100493

Karolina k, Bogusława L, Piotr PW. Isolation and determination of phenolic compounds from freshwater Cladophora glomerata. Algal Res. 2020 June; 48. 101912. https://doi.org/10.1016/j.algal.2020.101912

Beata M, Izabela M, Bogusława Ł, Grzegorz S, Bogusława G, Karolina K, et al. Valuable natural products from marine and freshwater macroalgae obtained from supercritical fluid extracts. Appl Phycol.2018 February; 30: 591–603. https://doi.org/10.1007/s10811-017-1257-5.

Aman G, Narendra KJ. Advances in green synthesis of nanoparticles. J Artif Cells Nanomed Biotechnol. 2019 Jan; 47(1): 844-851. https://doi.org/10.1080/21691401.2019.1577878

Abdelghany TM, Al-Rajhi AM, Al Abboud MA, Alawlaqi MM, Ganash Magdah A, Helmy EA, et al. Recent advances in green synthesis of silver nanoparticles and their applications: about future directions. Bionanoscience. 2018 March; 8(3): 5–16. https://doi.org/10.1007/s12668-017-0413-3.

Hamed M, Majid D. Zinc oxide nanoparticles: Biological synthesis and biomedical applications. Ceram Int. 2017 Jan; 43(1): 907-914. https://doi.org/10.1016/j.ceramint.2016.10.051

Imtiyaz H, Singh NB, Ajey S, Himani SS, Singh C. Green synthesis of nanoparticles and its potential application. Biotechnol Lett. 2016 April; 38:545–560. https://doi.org/10.1007/s10529-015-2026-7.

Babita B, Vivek P, Rajender SV. Glutathione promoted expeditious green synthesis of silver nanoparticles in water using microwaves. Green Chem. 2009 July ;11(7): 926–930.DIO: https://doi.org/10.1039/B900516A

Nadine W, Wolfgang T, Juergen B. Zinc oxide nanoparticles for therapeutic purposes in cancer medicine. J Mater Chem B. 2020 Jun 21; 8(23): 4973-4989. https://doi.org/10.1039/D0TB00739K.

Fatemeh M, Motahareh A, Alireza D, Zoleikha SJ. Antimicrobial Effect of Different Sizes of Nano Zinc Oxide on Oral Microorganisms. Front Dent. 2019 April 16(2): 105-112. https://doi.org/10.18502/fid.v16i2.1361

Jinhuan J, Jiang P, Jiye C. The Advancing of Zinc Oxide Nanoparticles for Biomedical Applications. Bioinorg Chem Appl. 2018 July; 18. https://doi.org/10.1155/2018/1062562.

Vishvanath T, Neha M, Keval GP, Solanki, NA, Monalisa T. Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii. Front Microbiol. 2018; 9: 1218. https://doi.org/10.3389/fmicb.2018.01218

Andrea F, Arianna B, Carlo P, Ilaria C, Iole V. Silver Nanoparticles for Water Pollution Monitoring and Treatments: Ecosafety Challenge and Cellulose-Based Hybrids Solution. Polymers. 2020 July; 12(8): 1635. https://doi.org/10.3390/polym12081635

Hosea M, Greene B, Mcpherson R, Henzl M, Alexander MD, Darnall DW. .Accumulation of elemental gold on the alga Chlorella vulgaris. Inorganica Chim Acta. 1986 March; 123(3): 161–165. https://doi.org/10.1016/S0020-1693(00)86339-2

Khwaja SS, Aziz UR, Tajuddin , Azamal H. Properties of Zinc Oxide Nanoparticles and Their Activity Against Microbes. Nanoscale Res Lett. 2018 May; 13: 14. https://doi.org/10.1186/s11671-018-2532-3

Chen X, Wu Z, Liu D, GAO Z. Preparation of ZnO photocatalyst for the efficient and rapid photocatalytic degradation of azo dyes. Nanoscale Res Lett. 2017 Feb.; 12(1): 1-10. https://doi.org/10.1186/s11671-017-1904-4

Seerangaraj V, Selvam S, Palanisamy S ,V.N. Kalpana, Govindaraju R, Mishal A, et al. Enhanced photocatalytic degradation of water pollutants using bio-green synthesis of zinc oxide nanoparticles (ZnO NPs). J Environ Chem . Eng. 2021 Aug; 9(4): 105772. https://doi.org/10.1016/j.jece.2021.105772

Naveed A, Syeda R R. Some members of ulotrichales from jalala, district mardan, pakistan. Pak J Pl Sci. 2009 Jan; 15(1): 19-30

Stein JR. Handbook of phycological method. Cambridge University press. Cambridge. 1975; 445 pp.

Shahin R, Nooshin N, Marwa B, Mohamed K, Anissa K, Ralf G. Water Extraction of Bioactive Compounds From Plants to Drug Development. Elsevier; 2017. 399-419 P. https://doi.org/10.1016/B978-0-12-809380-1.00016-4

Alharthi FA, Alghamdi AA, Alothman AA, Almarhoon ZM, Alsulaiman MF, Al-Zaqri N. Green Synthesis of ZnO Nanostructures Using Salvadora Persica Leaf Extract: Applications for Photocatalytic Degradation of Methylene Blue Dye. Crystal. 2020 May ; 10(6): 441. https://doi.org/10.3390/cryst10060441

Hong RY, Li JH, Chen LL, Liu DQ, Li HZ, Zheng Y, et al .Synthesis, surface modification and photocatalytic property of ZnO nanoparticles. Powder Technol. 2009 Feb.; 189(3): 426-432. https://doi.org/10.1016/j.powtec.2008.07.004

Shaoheng T, Jie Z. Antibacterial Activity of Silver Nanoparticles: Structural Effects. Adv Healthc Mater. May 2018; 7(13): 1701503. https://doi.org/10.1002/adhm.201701503.

Mounyr B, Moulay S, Saad KI . for in vitro evaluating antimicrobial activity. Pharm Anal. 2016 Apr; 6(2): 71–79. https://doi.org/10.1016/j.jpha.2015.11.005

Al-Kaisi KA. The genus Cyclotella Ktz. from some aquatic habitats in Iraq. Bull Sci. Baghdad. 1974; 15: 21-40.

Fouda A, Hassan SE, Saied E, Azab MS. An eco-friendly approach to textile and tannery wastewater treatment using maghemite nanoparticles (γ-Fe2O3 -NPs) fabricated by Penicillium expansum strain (K-w). J Environ Chem Eng. 2020; 9(1): 104693.

Ong WL, Natarajan S, Kloostra B, Ho GW. Metal nanoparticle-loaded hierarchically assembled ZnO nanoflakes for enhanced photocatalytic performance. Nanoscale.2013 Apr; 5(12): 5568. https://doi.org/10.1039/C3NR00043E

Zhang ZH, Xu Y, Ma XP, Li FY, Liu DN, Chen ZL, et al. Microwave degradation of methyl orange dye in aqueous solution in the presence of nano-TiO2-supported activated carbon(supported-TiO2/AC/MW). J Hazard Mater. 2012 March; 209: 271–277. https://doi.org/10.1016/j.jhazmat.2012.01.021.

Lan S, Liu L, Li RQ, Leng ZH, Gan SC. Hierarchical hollow structure ZnO: synthesis, characterization, and highly efficient adsorption/photocatalysis toward Congo red. Ind Eng Chem Res. 2014 Feb; 53(8): 3131–3139. https://doi.org/10.1021/ie404053m

Herrmann JM, Tahiri H, Ait-Ichou Y, Lassaletta G, Gonzalez-Elipe AR, Fernandez A. Characterization and photocatalytic activity in aqueous medium of TiO2 and Ag-TiO2 coatings on quartz. Appl Catal B: Environ. 1997 Nov.; 13(3-4): 219-228. https://doi.org/10.1016/S0926-3373(96)00107-5.

Kumar R, Kumar G, Umar A. ZnO nano-mushrooms for photocatalytic degradation of methyl orange. Mater Lett. 2013 Apr.; 97: 100–103. https://doi.org/10.1016/j.matlet.2013.01.044.

Hasnat MA, Siddiquey IA, Nuruddin A.Comparative photocatalytic studies of degradation of a cationic and an anionic dye. Dyes Pigm. 2005 Sep; 66(3): 185-188. https://doi.org/10.1016/j.dyepig.2004.09.020

Happy A, Soumya M, Venkat KS, Rajeshkumar S. Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route. Chem. Biol Interact. 2018 Apr; 286: 60–70. https://doi.org/10.1016/j.cbi.2018.03.008.

Padmavathy N, Vijayaraghavan R. Enhanced bioactivity of ZnO nanoparticles: An antimicrobial study. Sci Technol Adv Mater. 2008 Sep; 993): 035004. https://doi.org/10.1088/1468-6996/9/3/035004.

Sirelkhatim A, Mahmud S, Seeni A, Kaus NH, Ann LC, Bakhori SK, et al. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-Micro Lett. 2015 Apr; 7: 219–24

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