Green Synthesis of Silver Nanoparticles Using Aqueous Extract of Typha domingensis Pers. Pollen (qurraid) and Evaluate its Antibacterial Activity
Keywords:Antibacterial activity, Biogenic synthesis, AgNPs, aqueous extract, Typha domingensis
In this study, the aqueous extract of (Typha domingensis Pers.) pollen grain (qurraid) to know its ability to manufacture silver nanoparticles. Qurraid is a semi-solid yellow food substance, sold in Basra markets and eaten by the local population. It is made from the pollen of the T. domingensis Pers. plant after being pressed and treated with water vapor. The Gas chromatography–mass spectrometry (GC-MS) reaction was done to identify the active compounds of qurraid aqueous extract. The ability of the aqueous extract of qurraid to manufacture silver nanoparticles was tested, and the construction of silver nanoparticles was inferred by the reaction mixture's color, which ranged from yellow to dark brown. The synthesized silver nanoparticles (AgNPs) were described by UV-Vis, FTIR, XRD, SEM, and EDX. Then its anti-bacterial activity was estimated by the agar well diffusion method. The findings of the GC-MS analysis of the qurraid aqueous extract showed the major components with their ratio were: 5-Hydroxymethylfurfural with RT% 13.6196, 3-Deoxy-d-mannoic lactone 6.4285,. alpha.-L-lyxo-Hexopyranoside, methyl 3-amino-2,3,6-trideoxy- 4.264, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- 3.2078, and 1,3-Methylene-d-arabitol 3.1257. The construction of silver nanoparticles was described by spectroscopic methods, where the highest peak was recorded at 400nm by UV-Vis spectrum, which indicates the silver spectrum. The mineral nature of AgNPs was confirmed by XRD analysis, in which the highest peaks were, 111, 300, and 330 were recorded. In addition, the qrdAgNPs nanoparticles were spherical with sizes ranging from 20-70nm. The results of the EDX confirmed that the chemical composition of AgNPs was silver. The ability of the AgNPs was tested against four bacterial species, three of which were Gram-negative Escherichia coli A1, Escherichia coli A2, Alcaligenes faecalis AL1, and the fourth was Gram-positive bacteria Bacillus zanthoxyli B1 , which were identified by traditional and molecular methods using 16SrRNA gene sequencing, antibacterial activity results of AgNPs showed that it increases with increasing of AgNPs concentration, and the most sensitive species to silver particles was Alcaligenes faecalis AL1bacteria.
Published Online First 20/5/2023
Elegbede J, Lateef A, Nanotechnology in the built environment for sustainable development. IOP Conference Series: Mater Sci Eng. 2020; 805: 012044. https://doi.org/10.1088/1757-899X/805/1/012044
Ahmad SA, Das SS, Khatoon A, Ansari MT, Afzal M, Hasnain MS, et al.Bactericidal Activity of Silver Nanoparticles: A Mechanistic Review. Mater Sci Energy Technol. 2020; 3: 756-769. https://doi.org/10.1016/j.mset.2020.09.002
Bruna T, MaldonadoBravo F, Jara, P, Caro N. Silver nanoparticles and their antibacterial applications. Int J Mol Sci. 2021; 22, 7202. https://doi.org/10.3390/ijms22137202
Rai M K, Deshmukh, S D, Ingle A P, Gade A K. Silver nanoparticles: the powerful nano weapon against multidrug-resistant bacteria. Environ Res Health. 2012; 112(5): 841–852. https://doi.org/10.1111/j.1365-2672.2012.05253.x
Zulkifli NI, Muhamad M, Mohamad Zain NN, Tan WN, Yahaya N, Bustami Y, et al. A Bottom-Up Synthesis Approach to Silver Nanoparticles Induces Anti-Proliferative and Apoptotic Activities Against MCF-7, MCF-7/TAMR-1 and MCF-10A Human Breast Cell Lines. Molecules. 2020; 22; 25(18): 4332. https://doi.org/10.3390/molecules25184332
Petrucci OD, Hilton RJ, Farrer JK, Watt RK. A ferritin photochemical synthesis of monodispersed silver nanoparticles that possess antimicrobial properties. J Nanomater. 2019 (1): 1-8. https://doi.org/10.1155/2019/9535708
Parmar S, Kaur H, Singh J, Matharu AS, Ramakrishna S, Bechelany M.Advances in Green Synthesis of Ag NPs for Extenuating Antimicrobial Resistance. Nanomater. 2022 Mar 28; 12(7): 1115. https://doi.org/10.3390%2Fnano12071115
Song JY, Kim BS. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biocsyst Eng. 2009; 32 (1): 79. https://doi.org/10.1007/s00449-008-0224-6
Akintelu SA, Bo Y, Folorunso AS. A Review on Synthesis, Optimization, Mechanism, Characterization, and Antibacterial Application of Silver Nanoparticles Synthesized from Plants. J Chem. 2020; 1–12. https://doi.org/10.1155/2020/3189043
Townsend CC, Guest E. Flora of Iraq Volume 8 Monocotyledones (excluding Gramineae), Ministry of Agriculture of the Republic of Iraq, Richmond, Surrey : Royal Botanic Gardens, Kew 1966, 440pp.
Raji AI, Möller C, Litthauer D, van Heerden E, Piater LA. Bacterial diversity of biofilm samples from deep mines in South Africa. Biokemistri, 2008; 20 (2): 53-62. http://www.bioline.org.br/bk
Yassin MT, Ashraf AFM, Abdulaziz A A. In Vitro Evaluation of Biological Activities and Phytochemical Analysis of Different Solvent Extracts of Punica granatum L. (Pomegranate) Peels. Plants. 2021; 10 (12): 2742. https://doi.org/10.3390/plants10122742
Asmat-CamposD, Abreu AC, Romero-Cano MS, Urquiaga-Zavaleta J, Contreras-Cáceres R, Delfín-Narciso D, et al. Unraveling the Active Biomolecules Responsible for the Sustainable Synthesis of Nanoscale Silver Particles through Nuclear Magnetic Resonance Metabolomics. ACS Sustain Chem Eng. 2020; 8, 48: 17816–17827 https://dx.doi.org/10.1021/acssuschemeng.0c06903.
Ghosh G, Panda P, Rath M, Pal A, Sharma T, Das D. GC-MS analysis of bioactive compounds in the methanol extract of Clerodendrum viscosum leaves. Pharmacognosy Res. 2015 Jan-Mar;7(1):110-3. https://doi.org/10.4103/0974-8490.147223
Keerthiga M, Anand SP. Physicochemical, Preliminary Phytochemical Analysis and Antibacterial Activity agains Clinical Pathogens of Medicinally Important Orchid Geodorum densiflorum (Lam) Schltr. Int J Pharm Pharm Sci. 2014; 6(8): 558-61. https://journals.innovareacademics.in/index.php/ijpps/article/view/1925.
Bhalla N, Ingle N, Patri SV, Haranath D. Phytochemical analysis of Moringa Oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications. Saudi J Biol Sci. 2021; 2: 6-38. https://doi.org/10.1016/j.sjbs.2021.07.075
Pragatisheel, and J. Prakash. Silver Nanostructures, Chemical Synthesis Methods, and Biomedical Applications. In: Inamuddin, Asiri, A. (eds) Applications of Nanotechnology for Green Synthesis. (2020) Nanotechnology in the Life Sciences. Springer, Cham. https://doi.org/10.1007/978-3-030-44176-0_11
Afreen A, Ahmed R, Mehboob S, Tariq M, Alghamdi HA, Zahid AA, et al .Phytochemical-assisted biosynthesis of silver nanoparticles from Ajuga bracteosa for biomedical. Mater Res Expres. 2020; 7: 075404. https://doi.org/10.1088/2053-1591/aba5d0
Prakash O, Verma M, Sharma P, Kumar M, Kumari K, Singh A, et al. Polyphasic approach of bacterial classification — An overview of recent advances. Indian J Microbiol. 2007; 47(2): 98–108. https://doi.org/10.1007%2Fs12088-007-0022-x
Rahul M.Polyphasic systematics of marine bacteria and their alpha-glucosidase inhibitor activity. Ph.D. thesis. CSIR-National Chemical Laboratory, Pune-411 008, 2019, India.221pp. https://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/5847
P. Shanmuga Praba,P, Vasantha VS, Jeyasundari J, Jacob BA. Synthesis of plant-mediated silver nanoparticles using Ficus microcarpa leaf extract and evaluation of their antibacterial activities. Eur Chem Bull. 2015; 4(3): 117–120. https://doi.org/10.17628/ECB.2015.4.117-120
Anandalakshmi K, Venugobal J, Ramasamy,V. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Appl Nanosci. 2016; 6: 399–408. https://doi.org/10.1007/s13204-015-0449-z
Naseer QA, Xue X, Wang X, Dang S, Din SU, kalsoom , et al. Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential. Braz J Biol, 2021 Mar 5; 82. https://doi.org/10.1590/1519-6984.232434
Rautela A, Rani J, Debnath (Das) M. Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms. J Anal Sci Technol. 2019; 10: 1
Jemal K, Sandeep B V, Pola S. Synthesis, Characterization, and Evaluation of the Antibacterial Activity of Allophylus serratusLeaf and Leaf Derived Callus Extracts Mediated Silver Nanoparticles. J Nanomater. 2017:1–11. https://doi.org/10.1155/2017/4213275
Tufail MS, Liaqat I, Andleeb S, Naseem S, Zafar U, Sadiqa A, et al. Biogenic Synthesis, Characterization and Antibacterial Properties of Silver Nanoparticles against Human Pathogens. J Oleo Sci. 2022; 71, (2): 257-265. https://doi.org/10.5650/jos.ess21291
Femi-Adepoju A G, Dada A O, Otun KO, Adepoju AO, Fatoba OP. Green synthesis of silver nanoparticles using terrestrial fern (Gleichenia Pectinata (Willd.) C. Presl.): characterization and antimicrobial studies. Heliyon, 2019; 5(4): e01543. https://doi.org/10.1016/j.heliyon.2019.e01543
Hasson SO, Salman SAK, Hassan SF, Abbas SM. Antimicrobial Effect of Eco- Friendly Silver Nanoparticles Synthesis by Iraqi Date Palm (Phoenix dactylifera) on Gram-Negative Biofilm-Forming Bacteria. Baghdad Sci J. 2021; 18 (4): 1149. https://doi.org/10.3390%2Fijms23169257
Shareef AA, Hassan ZA, Kadhim MA, Al-Mussawi AA. Antibacterial Activity of Silver Nanoparticles Synthesized by Aqueous Extract of Carthamus oxycantha M.Bieb. Against Antibiotics Resistant Bacteria. Baghdad Sci.J. 2022;19(3): 460-468. https://doi.org/10.21123/bsj.2022.19.3.0460
Mikhailova EO. Silver Nanoparticles: Mechanism of Action and Probable Bio-Application. J Funct Biomater. 2020; 26; 11(4): 84 https://doi.org/10.3390%2Fjfb11040084
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