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Isolation and Identification of Polyethylene Terephthalate Degrading Bacteria from Shatt Al-Arab and Sewage Water of Basrah City


  • Eman Aboob Mukhaifi Department of Biology, College of Science, University of Basrah, Basrah, Iraq.
  • Hala Sabry Al-Atbi Department of Chemistry, College of Science, University of Basrah, Basrah, Iraq.
  • Suhyila Fadhil Ali Department of Biology, College of Science, University of Basrah, Basrah, Iraq.



Biodegradation, Degradation Bacteria, Klebsiella Pneumonia, Plastic, Polyethylene Terephthalate (PET).


Biodegradation is utilizing microorganisms to degrade materials into products that are safe for the
environment, such as carbon dioxide, water, and biomass. The current study aims to isolate and characterize
bacteria with polyethylene terephthalate (PET) degradation ability isolated from Shatt al-Arab water and
sewage from Basra, the bacteria were identified as Klebsiella pneumonia. According to the findings, the
isolates showed a highly significant difference in degradation of PET (24% during 7 days) and the percent of
degradation increased to 46% at 4 weeks compared to the control. The study also involved determining the
optimum temperature of K. pneumonia growth, which was 37°C, while the preferred pH was 7-8. The research
revealed that PET biodegradation by K. pneumonia can be used as a suitable and environmentally friendly


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