This is a preview and has not been published.

The combined effect of Weisella cibaria and Fusarium oxysporum nanoparticles on cervical cancer cells


  • Yusra MB. Muhsin Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
  • Huda Zuheir Majeed Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
  • Ali Murtatha Hasan Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
  • Sundus Qasim Mohammed Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
  • Nadia Zuhair Jassim Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.



Cervical cancer, Fusarium oxysporum, Lactic acid bacteria, Nanoparticles, Weissella cibaria


The predicted global cancer burden is expected to surpass 20 million new cancer cases by 2025. Despite recent advancements in tumor therapy, successful cancer treatment remains challenging. The emerging field of nanotechnology offers great opportunities for diagnosis, imaging, as well as treatment of cancer. The biosynthesis of silver nanoparticles by fungi is an ecologically clean and nontoxic method compared to other physical and chemical methods. The purpose of this study is to determine the Synergistic Effect of Combination Nanoparticles Synthesized from Fusarium oxysporum with Weisella cibaria against cervical cancer cells. The study has done from 2022 to March 2023 in the food microbiology laboratory in the Department of Biology / College of Science / Mustansiriyah University. Lactic acid bacteria (LAB) were isolated from food sources (Turnip, Cabbage, Cauliflower), after serial steps from treated NaCl , then cultured in MRS (Man-Rogosa-Sharpe) broth, finally examined under a microscope. The antibacterial activity of Cell Free Supernatant (CFS) s that was produced by these isolates was detected to choose the best one and diagnosed by PCR and DNA sequencing. The nanoparticles (AgNo3) that were produced from Fusarium oxysporum by biosynthesis were obtained from higher studies laboratory for fungi, and these fungi was submitted to toxicity test. The Synergist effect of chosen LAB and Fusarium oxysporum nanoparticles was studied against cervical cancer cells. Results show that all food sources were rich in LAB and the best antibacterial activity was to turnip source and according to molecular diagnosis was Weisella cibaria , that recorded in NCBI as (MG7865551). The synergistic effect of W. cibaria and nanoparticles showed and decrease the cancer line viability rate after 72 hr. exposure to this effect.


Download data is not yet available.


Torre LA , Islami F , Siegel RL , Ward EM , Jemal A. Global cancer in women: burden and trends global cancer in women: burden and trends .Cancer Epidemiol Biomarkers Prev . Apr 2017; 26(4): 444-457. Epub 2017 Feb 21.

Ahmed AA , Hamzah H , Maaroof M. Analyzing formation of silver nanoparticles from the filamentous fungus Fusarium oxysporum and their antimicrobial activity. Turkish J Biol. Feb 2018; 42(1): 54-62. eCollection 2018.

Salazar L, López M, Grijalva M, Castillo L, Maldonado A. Biological effect of organically coated Grias neuberthii and Persea americana silver nanoparticles on HeLa and MCF-7 cancer cell lines. J Nanotech Aug 2018; 26(4): 444-457 .

Shahzad A, Saeed H, Iqtedar M, Hussain SZ, Kaleem A, Abdullah R, Sharif S, Naz S, Saleem F, Aihetasham A, Chaudhary A. Size-controlled production of silver nanoparticles by Aspergillus fumigatus BTCB10: likely antibacterial and cytotoxic effects. J Nanomat. Feb 2019; 1-14. Article ID 5168698 .

Korbekandi H , Ashari Z , Iravani S and Abbasi S. Optimization of biological synthesis of silver nanoparticles using Fusarium oxysporum. Iran J Pharma Res. Jul 2013; (12)3: 289-98.

Wang Q, Li S, Zhao F, Dai H, Bao L, Ding R. Chemical constitutes from endophytic fungus Fusarium oxysporum. Fitoterapia. 2011; 82(5): 777-81.

Lee KW, Park JY, Jeong HR, Heo HJ, Han NS, Kim JH. Probiotic properties of Weissella strains isolated from human feces. Anaerobe. Dec 2011; 18(1): 96-102

Kang MS , Yen JE , Oh J , Shin B, Kim J. Complete Genome sequence of Weisella cibaria strains CMU, CMS1, CMS2 and CMS3 isolated from infant saliva is South Korea. American Society for Microbiology. Genome Announc .Oct 2017; 5(40): e01103-17.

Ojekunle O, Banwo K, Sanni AI. In vitro and in vivo evaluation of Weissella cibaria and Lactobacillus plantarum for their protective effect against cadmium and lead toxicities. Lett Appl Microbiol. May 2017; 64(5): 379-385.

Björkroth KJ, Schillinger U, Geisen R, Weiss N, Hoste B, Holzapfel WH, Korkeala HJ, Vandamme P. Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples. Int J Sys Evol Microbiol. 2002 Jan; 52(1): 141-8.

Mohsen YM, Shawket DS, Abd-AlSattar D. Novel Probiotic Bifidobacterium overcomes synergistic effect of three natural biotic OMNI Drug and antibiotic against some UTI pathogens. Int J Sci Nature. Jan 2013; 4(3): 456-462.

Askoul I , Gorrah S, Al-Amir L. Isolation and characterization of lactobacilli bacteria from some Syrian foods and detection the effectiveness of antipathogenic bacterial growth, Damascus J Agricul Scien . Feb 2014 ; 30(1): 227-237.

Holzapfel WH , Wood BJB. Lactic Acid Bacteria , Biodiversity and Taxonomy. John Wiley and Sons Ltd. UK. Apr 2014.[cited 2022 Nov2] .85p.

Abbas HH , Abd-Mohammed SA , Shawkat DS , Baker YM. Effect of Lactobacillus sp. Crude Bacteriocin (CB) and Cell-Free Supernatant (CFS) against E. coli growth and adherence on vaginal epithelial cell surface. Inter J Advan Res. Jan 2016; 1(1): 614-620.

Jasim EI, Shawket DS, Mohsin YMB, Mohammed BB, Ibraheem D A, Hussein MA. Comparative study between the effect of the Lactic Acid Bacteria and non against some pathogenic bacteria . Inter J Advan Biol Res. Mar 2017; 7(4): 656-660.

Rahi GK , Ajah HA. Antagonistic activity of silver nanoparticles synthesis by Fusarium oxysporum against Candida spp. Pak J Biotechnol. June 2018; 15(2): 347-356.

Elavarasi V, Pugazhendhi A, Poornima TK, Valsala H , Thamaraiselvi K. Screening and characterization of Weissella cibaria isolated from food source for probiotic properties . Int J Comp Appl. Jan 2014; 1: 29-32.

Kamboj K, Vasquez A, Balada-Llasat JM. Identification and significance of Weissella species infections. Front Microbiol. 2015 Oct 31; 6: 1204.

Mohsin YMB, Hasan AM. Dari WA. Natural product of Lactococcus overcome nosocomial infection in some of Baghdad hospitals in Iraq. Baghdad Sci J. Mar 2020; 1791: 227-234.

Muhsin YMB , Majeed HZ. , Shawkat, D.S. CFS and crude bacteriocin of Lactococcus against growth and biofilm formation of some pathogenic bacteria. Int J Curr Micro App Sci.

Abdulhadi AS, Shafiq SA Mohsen YMB. Cytotoxic activity of partial purified proteins Saccharomyces and Lactic acid bacteria on HT-29 cell line. Ann Romanian Soc Cell Biol. June 2021; 25(7): 1670-1679.

Penido FC, de Oliveira Goulart C, Galvão YC, Teixeira CV, de Oliveira RB, Borelli BM, et al. Antagonistic lactic acid bacteria in association with Saccharomyces cerevisiae as starter cultures for standardization of sour cassava starch production. J Food Sci Technol . 2019 Sep 1; 56(9): 3969-79.

Ali EN, Kadhem AA, Majeed HZ Mohsin YMB. Immunomodulatory effects of Pediococuus acidilactici on BALB/C mice immunosuppressed by cyclophosphamide. Res J Pharma Tech. Nov 2022; 15(12): 605-610.

Ahmed S, Singh S, Singh V, Roberts KD, Zaidi A, Rodriguez-Palacios A. The Weissella genus: Clinically treatable bacteria with antimicrobial/probiotic effects on inflammation and cancer. Microorganisms. 2022 Dec 7; 10(12):2427.

Shatti ZO, Mohammed BB Mohsin YMB .Four screening methods to determine Pediococcus acidilactici efficacy against two biofilm producing pathogenic bacteria., Ind J Publ Health Res. Apr 2020; 10(10): 3810-3814

Mohsin YMB, Majeed HZ Mohammed BB. Lactic acid bacteria biosurfactant role that isolated from human breast milk in inhabit eyes pathogenic bacteria. Ibn-Al-Haitham J Pure. Appl. Sep 2018; 31(2): 31-40.

Mostafa F. Biosynthesis of silver nanoparticles by pathogenic and nonpathogenic strains of Fusarium oxysporum . sp. lycopersici. Egypt J Bot. Aug 2017; 57(2) : 345-350.

Korbekandi H, Ashari Z, Iravani S, Abbasi S. Optimization of biological synthesis of silver nanoparticles using Fusarium oxysporum. Iran J Pharma Res .Summer 2013; 12(3): 289 -298.

Barik BP , Tayung K, Jagader P , Dutta SK. Phylogenetic Placement of an Endophytic Fungus Fusarium oxysporum Isolated from Acorus calamus Rhizomes with Antimicrobial Activity. Eur J Bio Sci. Jan- March 2010; 2(1): 8-16.

Ishida K , Cipriano TF , Rocha GM , Weissmuller G, Gomes F, Miranda K, et al. Silver nanoparticles production by the fungus Fusarium oxysporum: nanoparticle characterization and analysis of antifungal activity against pathogenic yeasts. Mem Inst Oswaldo Cruz, Rio de Janeiro , Apr 2014; 109(2): 220-8. 0276130269.

Park JH , Ahn HJ , Kim SG, Chung CH. Dextran-like exopolysaccharide-producing Leuconstoc and Weissella from kimchi and its gradient. Food Sci Biotechnol. Aug 2013; 22: 1047- 1053.

Kwak S, Co Y, Noh G, Om A. Cancer preventive potential of kimchi lactic acid bacteria (W. cibaria, L. plantarum). J Cancer Prev. Dec 2014; 19 (4): 253-258.

Husseiny SM , Salah TA , Anter H A. Biosynthesis of size controlled silver nanoparticles by Fusarium oxysporum, their antibacterial and antitumor activities .J Basic Appl Sci. Sep 2015; 4: 225-231.

Jalgaonwala R ,Mahajan RT. Production of anti-cancer enzyme asparaginase from endophytic Eurotium sp. isolated from rhizomes of Curcuma longa. Eur J Exp Biol . Oct 2014; 4(3): 36-43.

Yadav N, Surkar S. Production of asparaginase by Fusarium oxysporum using submerged fermentation. Int J Pharma Sci Invent. June 2014; 3(6): 32-40.

Chow YY, Ting ASY. Endophytic L-asparaginase producing fungi from plants associated with anticancer properties. J Adv Res. Nov 2015; 6(6): 869-867.

Auda IG, Mohsin YM, Jasim EI ,Sharan Z. Ouda JG. Partial sequencing ISI2I6V transpsase gene of Staphylococcus aureus isolated from samples , Al-Kindy Coll Med J. Jan 2018; 14(2): 24- 26.

Abdel-Rahman TM , Khalil NM, El-Ghany MN , Yosef E. Purification , characterization and medicinal application of tyrosinase extracted from Saccharomyces cerevisiae . J Innov. Pharm Biol Sci. Jan 2019; 6(1): 1-11.

Al-Kassie G, Al-jumaa Y, Jamel Y. Effect of probiotic (Aspergillus niger) and prebiotic (Taraxacum officinale)on blood picture and biochemical properties of broiler chicks. Int J Poult Sci. July 2018; 7.(12): 1182-1184.

Al-Shammari AM, Al-Saadi H, Al-Shammari SM, Jabir MS. Galangin enhances gold nanoparticles as anti-tumor agents against ovarian cancer cells. AIP Conf Proce .Mar 25 2020; 2213(1): 020206-1–020206-7.AIP Publishing.

Haryani Y, Halid NA, Guat GS, Nor-Khaizura MA, Hatta A, Sabri S, et al. Characterization, molecular identification, and antimicrobial activity of lactic acid bacteria isolated from selected fermented foods and beverages in Malaysia. FEMS Microbiol. Lett. 2023; 370: fnad023.

Haryani Y, Halid NA, Guat GS, Nor-Khaizura MA, Hatta A, Sabri S, et al. Characterization, molecular identification, and antimicrobial activity of lactic acid bacteria isolated from selected fermented foods and beverages in Malaysia. FEMS Microbiol. Lett. 2023; 370: fnad023.

Musah SS, Owuna JE, Makut MD, Adamu BB, GI I, Izebe K, et al. Antibacterial activity of lactic acid bacteria isolated from Etsako Osuemegbe rice. AROC Food Nutr. 2023; 2(1): 01-05.

Bartkiene E, Lde V Ruzauskas M. Lactic acid bacteria isolation from spontaneous sourdough and their characterization including antimicrobial and antifungal properties evaluation. Microorganisms. Dec 2020; 8(1): 64

Walia S, Kamal R ,Kanwar SS. Chemoprevention by Probiotic during 1,2-Dimethyl hydrazine induced colon carcinogenesis. Dig Dis Sci Apr 2018: 63(4): 900-909. 018-4949-z.

Kiczorowski P , Kiczorowski B, Mieczan A. Effect of fermentation of chosen vegetables on the nutrient , mineral and biocomponent profile in human and animal nutrition. Sci Rep. Aug 2022; 12: 13422.

Junnarkar M ,Pawar S, Gaikwad S. Nawani N. Probiotic Potential of Lactic Acid Bacteria from fresh vegetables : Application in food preservation .Ind J Exp Biol. Oct 2019; 57 : 825-838.

Poore J. Nemecek T. Reducing foods environmental impact through producers and consumers. Science. Jun 2018; 360 : 987-992 .

Joch M, Kudrana V. Hucko B. Effect of Geraniol and Camphene on in vitro rumen fermentation and methane production . Sci Agric Bohem. Jan 2017; 48: 63-69. https://doi.10.1515/sab-2017-0012.

Grenda T, Grenda A, Domaradzki P, Kwiatek K. Probiotic potential of Clostridium spp. advantages and Doubts .Curr Issues Mol .Biol .Jul 2022; 44 (7): 3118-3130.

Manik M, Kaban J, Silalahi J Gimting M. Lactic Acid Bacteria (LAB) with probiotic potential from Dengka Naniura. Baghdad Sci J .Mar 2021; 18 (1): 35-40.