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Studying Myostatin as a Diagnostic Indicator in Sera of Iraqi Women with Metabolic Syndrome


  • Riyam Hussein Assaf Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq.
  • Layla Othman Farhan Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq.



Cholesterol, Fasting blood Glucose, Myostatin, Metabolic Syndrome, Low-density lipoprotein


Obesity in the abdominal area, insulin resistance, hyperglycemia, dyslipidemia, and hypertension are all included as part of metabolic syndrome. Myocytes manufacture and release the myokine known as myostatin, which acts on muscle cells inhibiting cellular growth. This study aimed to compare Myostatin along with other biochemical parameters (Fasting blood sugar (FBS), cholesterol, high-density lipoprotein (HDL), triglycerides (T.G), low-density lipoprotein (LDL), and very Low-density lipoprotein (VLDL)) in sera of Iraqi with metabolic syndrome patients and healthy subjects. Eighty women were separated into two groups: group I, 40 metabolic disorders patients, and group II, 40 healthy people, which served as the control group. Myostatin levels significantly increased compared to the control group in the group with metabolic syndrome, according to the data p > 0.001. A definite cut-off value was visible on the Myostatin ROC curve (5), in the metabolic syndrome. The outcomes suggest myostatin as possible diagnostic tool for metabolic syndrome.


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Saltiel AR, Kahn CR. Insulin signalling and the regulation of glucose and lipid metabolism. Nature. 2001;414(6865):799–806.

Hameed EK, Al-Ameri LT, Hasan HS, Abdulqahar Z. The Cut-off Values of Triglycerides-Glucose Index for Metabolic Syndrome Associated with Type 2 Diabetes Mellitus. Baghdad Sci J. 2021; 19(2): 340–6.

Ormazabal V, Nair S, Elfeky O, Aguayo C, Salomon C, Zuñiga FA. Association between insulin resistance and the development of cardiovascular disease. Cardiovasc. Diabetol. 2018; 17(1): 1–14.

Mehdi WA, Jassim RA. Synthesis of silver nanoparticles from leaf extract of olive and fig with silver nitrate and effect on ecto-5’-nucleotidase (5’-nt), ada and ampda enzymes in sera of arthrosclerosis patients. Int J Chem Sci. 2016; 14(3): 1805-1817.

Abed BA, Hamid GS. Evaluation of Lipocalin-2 and Vaspin Levels in In Iraqi Women withType 2 Diabetes Mellitus. Iraqi J Sci. 2022;63(11) :4650–8.

Ishimaru M, Matsui A, Seki K, Korosue K, Akiyama K, Mizukami H, et al. Effects of different winter climates in Japan on body composition of young Thoroughbreds in training. J Vet Med Sci. 2022;84(12) 22–378.

Ahmadi MN, Pfeiffer KA, Trost SG. Physical activity classification in youth using raw accelerometer data from the hip. Meas Phys Educ Exerc Sci. 2020; 24(2): 129–36.

Ibrahim SA, Zainulabdeen JA, Jasim HM. The significance of spermidine and spermine in association with atherosclerosis in sera of Iraqi patients. Biomed Pharmacol J. 2018; 11(3): 1389–96.

Haddad NI, Nori E, Hamza SA. Correlations of Serum Chemerin and Visfatin with other Biochemical Parameters in Iraqi Individuals with Metabolic Syndrome and Type Two Diabetes Mellitus. Jordan J Biol Sci. 2018; 11(4): 1-15.

Ibrahim SA, Jasim HM, Zainulabdeen JA. Association of Arginase I Gene Polymorphism with the Risk of Atherosclerosis in a Sample of Iraqi Patients. Indian J Public Heal Res Dev. 2019; 10(6): 526–531.

Abd Al-Ghanny RJ, Al-Moosawi MMB, Abd BA. Effects of Vitamin D Deficiency in Polycystic Ovarian Syndrome. Iraqi J Sci. 2022; 63(1): 33–42.

Hittel DS, Berggren JR, Shearer J, Boyle K, Houmard JA. Increased secretion and expression of myostatin in skeletal muscle from extremely obese women. Diabetes. 2009; 58(1): 30–8.

Abed BA, Al-AAraji SB, Salman IN. Estimation of galanin hormone in patients with newly thyroid dysfunction in type 2 diabetes mellitus. Biochem Cell Arch. 2021 ; 21(1) :1317-1321,

Feig DS, Donovan LE, Zinman B, Sanchez JJ, Asztalos E, Ryan EA, et al. Metformin in women with type 2 diabetes in pregnancy (MiTy): a multicentre, international, randomised, placebo-controlled trial. Lancet Diabetes Endocrinol. 2020; 8(10): 834–44.

Farhan LO, Taha EM, Farhan AM. A Case control study to determine Macrophage migration inhibitor, and N-telopeptides of type I bone collagen Levels in the sera of osteoporosis patients. Baghdad Sci J. 2022; 19(4); 848-854.

Pervin S, Reddy ST, Singh R. Novel roles of follistatin/myostatin in transforming growth factor-β signaling and adipose browning: Potential for therapeutic intervention in obesity related metabolic disorders. Front Endocrinol (Lausanne). 2021; 12(4): 1-17.

Muramatsu H, Kuramochi T, Katada H, Ueyama A, Ruike Y, Ohmine K, et al. Novel myostatin-specific antibody enhances muscle strength in muscle disease models. Sci Rep. 2021; 11(1): 1–16.

Abass EA, Abed BA, Mohsin SN. Study Of Lysyl Oxidase-1 And Kidney Function In Sera Of Iraqi Patients With Diabetic Nephropathy. Biochem Cell Arch. 2021 ; 21( 1): 1129-1132

Goodger R, Singaram K, Petrov MS. Prevalence of Chronic Metabolic Comorbidities in Acute Pancreatitis and Its Impact on Early Gastrointestinal Symptoms during Hospitalization: A Prospective Cohort Study. Biomed hub. 2021; 6(3): 111–7.

Khan SA, Ram N, Masood MQ. Patterns of abnormal glucose metabolism in acromegaly and impact of treatment modalities on glucose metabolism. Cureus. 2021; 13(3).

Preda SA, Comanescu MC, Albulescu DM, Dascălu IT, Camen A, Cumpătă CN, et al. Correlations between periodontal indices and osteoporosis. Exp Ther Med. 2022; 23(4): 1–7.

Grillo A, Colapietro N, Salvi P, Furlanis G, Baldi C, Rovina M, et al. Estimation of mean arterial pressure by the analysis of brachial pulse waveform recorded by applanation tonometry and comparison with currently used algorithms. J Hypertens. 2021; 39(4): 125–136.

Mehdi WA, Farhan LO, Abed BA. Biochemical and Kinetic Studies on Alkaline Phosphatase and other Biochemical Features in Sera of Patients with type 2 Diabetes. Baghdad Sci J. 2012; 9(1): 160-167.

Zainulabdeen JA. Is serum amylase normal in women with polycystic ovarian syndrome. Baghdad Sci J. 2014; 11(4): 1583-1591.

Baumgartner M, Lischka J, Schanzer A, de Gier C, Walleczek N-K, Greber-Platzer S, et al. Plasma Myostatin Increases with Age in Male Youth and Negatively Correlates with Vitamin D in Severe Pediatric Obesity. Nutrients. 2022; 14(10): 2-10.

Tahir NT, Abdulsattar SA, Alkazzaz FF. Assessment of Obesity, Dyslipidemia, Hyperglycemia, and Pro-Inflammatory Cytokines as Cardiovascular Disease Risk Factors in Acromegaly Patients. Baghdad Sci J. 2022; 19(5): 976-980;

Han D-S, Chu-Su Y, Chiang C-K, Tseng F-Y, Tseng P-H, Chen C-L, et al. Serum myostatin is reduced in individuals with metabolic syndrome. PLoS One. 2014; 9(9): 1-7.

Allen DL, Hittel DS, McPherron AC. Expression and function of myostatin in obesity, diabetes, and exercise adaptation. Med Sci Sports Exerc. 2011; 43(10): 1828-1835.

Wang F, Liao Y, Li X, Ren C, Cheng C, Ren Y. Increased circulating myostatin in patients with type 2 diabetes mellitus. J Huazhong Univ Sci Technol (Medical Sci). 2012; 32(4): 534–539.

Baig MH, Ahmad K, Moon JS, Park S-Y, Ho Lim J, Chun HJ, et al. Myostatin and its Regulation: A comprehensive review of myostatin inhibiting strategies. Front Physiol. 2022; 2391: 1-16.

Lebrasseur NK. Building muscle, browning fat and preventing obesity by inhibiting myostatin. Diabetologia. 2012; 55(1): 13–7.

Wojcik S, Nogalska A, Engel WK, Askanas V. Myostatin and its precursor protein are increased in the skeletal muscle of patients with Type-II muscle fibre atrophy. Folia Morphol (Warsz). 2008; 67(1): 8–14.