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Evaluation of the Potential Protective Role of Galangin Associated with Gold Nanoparticles in the Histological and Functional Structure of Testes of Adult Male Albino Mice Administrated with Carbon Tetrachloride


  • Bassem Sultan Abeed Department of Biology, College of Education for Pure Sciences/Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq.
  • Hanady Salim Al-Shmgani Department of Biology, College of Education for Pure Sciences/Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq.



albino mice, CCl4, Galangin, Gold nanoparticles, testicular tissue


Galangin (Gal) is a natural flavonoid sourced from the roots of the plant Albina Kalanga. It possesses a variety of pharmacological activities, such as antioxidant, anticancer, and anti-inflammatory. The current study aims to enhance the efficacy of galangin through the use of gold nanoparticles as a drug delivery system against CCl4-induced toxicity in the testicular tissue of male albino mice. Forty-two albino mice were divided into seven groups (6 mice/group), treatment with carbon tetrachloride solution for two weeks by intraperitoneal injection, (1 ml/kg) once a week for all groups except the control group, after which a group was injected with gold nanoparticles and two groups were injected with galangin in two concentrations, and two groups were injected with AuNPs + Gal conjugation solution with two concentrations. The animals' bodies were weighed and blood samples were obtained for testosterone hormone analysis and testicles for the purpose of weighing and histological study at the end of the experiments. The study showed that the testicular tissue of mice treated with CCl4 had a different pathology compared to the control group, the testosterone hormone levels in the CCl4 group were significantly higher than those of the control group. Results from the AuNPs+Gal group showed a significant reduction in the effects of CCl4 toxicity on mice testicles, with testosterone levels are returning to normal and histological testicle structures improving, the ratio of testicle weights to animal body weight in the group injected with AuNPs was significantly higher than that of the control and CCl4 groups. In conclusion, the results revealed that galangin combined with gold nanoparticles could effectively reduced the histological and functional tissue damages caused by CCl4, presenting a promising natural solution that needs to be further developed


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Unsal V, Cicek M, Sabancilar İ. Toxicity of carbon tetrachloride, free radicals and role of antioxidants. Rev Environ Health . 2021; 36(2): 279-295.‏ .

Duan Z, Zhang Y, Zhu C, Wu Y, Du B, Ji H. . Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice. Int J Biol Macromol, 2020; 162: 533-547.‏ .

Zhang X, Kuang G, Wan J, Jiang R, Ma L, Gong X, et al. Salidroside protects mice against CCl4-induced acute liver injury via down-regulating CYP2E1 expression and inhibiting NLRP3 inflammasome activation. Int immunopharmacol, 2020; 85: 106662.‏

Sattar A H. Fundamentals of pathology: medical course and step 1 review. Pathoma, 2019.‏

Al-Shmgani HS, Kadri ZH, Al-Halbosiy MM, Dewir YH. Phytochemical analysis, cytotoxicity and antioxidant activity of cuckoo pint (Arum maculatum) leaf extract. Acta Biol Szeged . 2019; 63(2): 119- 24. .

Anwar SS, Al-Shmgani HS, Tawfeeq AT, Sulaiman GM, Al-Mousawi YH. In silico analysis of quercetin as a potential anti-cancer agent. Mater Today: Proc. 2021; March 30; 42(5): 2521-2526.

Patil S, Ujalambkar V, Rathore A, Rojatkar S, Pokharkar V. Gal-loaded galactosylated pluronic F68 polymeric micelles for liver targeting. Biomed Pharmacother. 2019; Apr; 112: 108691. .

Singh D, Saini A, Singh R , Agrawal R. Galangin, as a Potential Anticancer Agent. Rev Bras Farmacogn..2022; 32(3) : 331-343.‏ .

Hadi IS, Yas RM. Gamma Radiation Effect on Characterizations of Gold Nanoparticles Synthesized Using Green Method. Iraqi J Sci. 2022; 4305-4313.‏ 10.24996/ijs.2022.63.10.17 .

Qaddoori M H, Al-Shmgani HS. Hematological, biochemical, and DNA analysis of Gal immobilized on AuNPs. Ann For Res. 2022; 65(1): 3984-3999.

Sani MJ. Spin-orbit coupling effect on the electrophilicity index, chemical potential, hardness and softness of neutral gold clusters: A relativistic ab-initio study. HighTech Innov J. 2021; 2(1): 38-50.‏

Qaddoori M H, Al-Shmgani H S. Galangin-Loaded Gold Nanoparticles: Molecular Mechanisms of Antiangiogenesis Properties in Breast Cancer. Int J Breast Cancer. 2023; 2023, Article ID 3251211: 14 pages. .

Kien P H, Khamphone Y, Trang GTT. Study of effect of size on iron nanoparticle by molecular dynamics simulation. High Tech Innov J. 2021; 2(3): 158-167.‏

Sulaiman GM, Waheeba HM, AL-Shmgani H, Eassa HA, Al-Amiery AA, Jabir MS, et al. Synthesis, Molecular Modeling, DNA Damage Interaction, and Antioxidant Potential of Hesperidin Loaded on Gold Nanoparticles. J Biomim Biomater Biomed Eng. 2022; 5417-29.

Abeed BS, Al-Shmgani HS, Khalil KAA, Mohammed HA. Histological and Biochemical Effect of Galangin Conjugated Gold Nanoparticles on the kidneys Damage Induced by Carbon Tetrachlorideof Adult Male Albino Mice. Ibn AL-Haitham J Pure Appl Sci, , 2023 July; 36(3):72-84. /10.30526/36.3.3250.

Al-Musawi MM , Al-Bairuty GA , Al-Shmgani, HS. The Comparative Effect of Copper Oxide Nanoparticles and Copper Sulfate on Reproductive Hormones and Sperm Parameters in Mature Male Albino Mice. Ann Biol. 2022; 38( 2). 317–321.

AL-Musawi MS, Al-Shmgani HS, Al-Bairuty GA. Histopathological and Biochemical Comparative Study of Copper Oxide Nanoparticles and Copper Sulphate Toxicity in Male Albino Mice Reproductive System. Int J Biom. 2022 May; 16( 2022).12 page.

Abukhalil MH, Althunibat OY, Aladaileh S H, Al-Amarat W, Obeidat H M, Alayn'Al-marddyah A, et al. Galangin attenuates diabetic cardiomyopathy through modulating oxidative stress, inflammation and apoptosis in rats. Farmacognosia.2021;1 38: 111410.‏

Abd_Alkazem D M, Rabee AM.The Effect of Endosulfan Pesticide in Some Biochemical Parameters of White Mice. Ibn Al-Haitham J Pure Appl Sci. 2023; 36(1) : 36-47. ‏ .

Abdul-Lattif R F. Effect of sesame oil on lipid profile and liver enzymes in male albino rats treated with carbon tetrachloride (CCl4). Ibn AL-Haitham J Pure Appl Sci. 2018;Special issue: 41-48.‏ 1769 .

Luaibi NM , Mohammed RA . Physiological and Hormonal Effects of Titanium Dioxide Nanoparticles on Thyroid Function and the Impact on Bodyweight in Male Rats. Baghdad Sci J. 2023; 20(2): 0416-0416.‏ .

Bayram EN, Al-Bakri NA, Al-Shmgani HS. Zinc Chloride Can Mitigate the Alterations in Metallothionein and Some Apoptotic Proteins Induced by Cadmium Chloride in Mice Hepatocytes: A Histological and Immunohistochemical Study. J Toxico.2023; 2023, Article ID 2200539: 8 pages. .

Haddad B S, Hassan K K. Synthesis and Characterization of New Selenonitrone Derivative and Its Effect on Breast Cancer Cell Line Viability in Vitro. Baghdad Sci J. 2019 Sep; 16(3 Suppl.): 0754-0763 .

Chatterjee A. Testicular degeneration in rats by carbon tetrachloride intoxication. Experientia(Basel). 1966; 226: 395–396.

Hashem AS. Defensive impact of propolis against CCl4 actuated rats’ testicular damage. J Adv Vet Anim Res. 2021; 8(1): 70–77. .

Sanderson JT. The steroid hormone biosynthesis pathway as a target for endocrine-disrupting chemicals. Toxico sci. 2006; 94(1): 3-21.‏ .

Jana K, Samanta PK, Manna I, Ghosh P, Singh N, Khetan RP, et al., Protective effect of sodium selenite and zinc sulfate on intensive swimming-induced testicular gametogenic and steroidogenic disorders in mature male rats. Appl Physiol. 2008; 33(5): 903-914.‏ .

Zou P, Wang X, Yang W, Liu C, Chen Q, Yang H, et al., Mechanisms of stress-induced spermatogenesis impairment in male rats following unpredictable chronic mild stress (uCMS). Int J mol sci. 2019; 20(18): 4470.‏ .

Abeer ME, Amal AS, Faiza AM. The potential protective effect of propolis on experimentally induced hepatitis in adult male albino rats. histological and immunohistochemical study. J Histol Histopathol 2015; 2(4): 1–9. .

Kapoor D, Singh S, Kumar V, Romero R, Prasad R, Singh, J. Antioxidant enzymes regulation in plants in reference to reactive oxygen species (ROS) and reactive nitrogen species (RNS). Plant Gene. 2019; 19: 100182. .

Sadiq KF, Mahmood H S. Enhancement of Antibacterial Activity of Face Mask with Gold Nanoparticles. Ibn AL-Haitham J Pure Appl Sci. 2022; 35(3): 25-31.‏ .

Fidan AF, Dundar Y. The effects of Yucca schidigera and Quillaja saponaria on DNA damage, protein oxidation, lipid peroxidation and some biochemical parameters instreptozotocin-induced diabetic rats. J Diabetes Complicat. 2008; 22(5): 348–56.

Hashem A S. Defensive impact of propolis against CCl4 actuated rats’ testicular damage. J Adv Vet Anim Res. 2021; 8(1): 70.‏ .