التصنيع الحيوي لدقائق الفضة النانوية باستخدام الأيوض الخارج خلوية للبكتيريا البحريةKocuria flava واختبار دورها في تعزيز الفعّالية الضد ميكروبية للسيبروفلوكساسين
DOI:
https://doi.org/10.21123/bsj.2024.9965الكلمات المفتاحية:
AgNPs, Antibiotic enhancement, Ciprofloxacin, Kocuria flava, MDRالملخص
تهدف الدراسة الحالية إلى تصنيع دقائق الفضة النانوية (AgNPs) حيوياً باستخدام الأيوض الخارج خلوية للبكتيريا البحرية Kocuria flava (F57)، وأستخدامها لتعزيز فعّالية المضاد الحيوي السيبروفلوكساسين تجاه البكتيريا المرضية ذات المقاومة المتعددة للمضادات الحيوية (MDR). جُمعت عينّات المياه البحرية من المياه البحرية العراقية خلال شهر كانون الثاني/2022. تم تشخيص العزلةF57 من خلال الصفات المجهرية، وبعض الاختبارات الكيموحيوية، والتشخيص الجزيئي بتضخيم الجين 16S rDNA، وظهر من خلال رصف تسلسلات القواعد النايتروجينية للجين 16S rDNA مع تلك العائدة للسلالات المسجّلة في بنك الجينات NCBI، وشجرة النشوء والتطور، وجود تطابق نسبته 99.93% مع السلالة K. flava AUMC B-459. أظهرت نتائج قياس الطيف الكتلي GC/MS لمستخلص العزلة F57 وجود ثلاثين مركباً. أُستخدمت الأيوض الخارج خلوية للعزلة F57 لتصنيع دقائق الفضة النانوية، وتم التأكد من نجاح عملية تصنيع الحيوي عن طريق التحليل الطيفي للضوء المرئي- الأشعة فوق البنفسجية وطيف الأشعة تحت الحمراء وحيود الأشعة السينية والمجهر الإلكتروني الماسح وطاقة تشتت طاقة الأشعة السينية. أظهرت دقائق الفضة النانوية فعّالية تجاه خمس عزلات من البكتيريا المرضية ذات المقاومة المتعددة للمضادات الحيوية وهي كلٌ من:Klebsiella pneumoniae ، Pseudomonas aeruginosa ، Staphylococcus haemolyticus ، وعزلتان من البكتيريا Escherichia coli (1&2)، تم تحديد التركيز المثبّط الأدنى لدقائق الفضة النانوية والمضاد الحيوي Ciprofloxacin ومزيجهما معاً ضد العزلات البكتيرية المرضية أعلاه، وكانت العزلة P. aeruginosa هي الأكثر حساسية لدقائق الفضة النانوية، إذ كان التركيز المثبطّ الأدنى تجاهها 7.81 مايكروغرام/مل، وكانت جميع العزلات البكتيّرية المرضية مقاومة للمضاد الحيوي Ciprofloxacin، وأظهرت نتائج مزج المضاد الحيوي Ciprofloxacin مع دقائق الفضة النانوية وجود تأثير تآزري تجاه العزلات المرضية P. aeruginosa و S. haemolyticus وE. coli (2) ، وأصبحت العزلتين E. coli (1&2) حساسة لهذا المضاد الحيوي بعد عملية المزج. لذا فإن دقائق الفضة النانوية المخلّقة حيوياً بإستخدام الأيوض الخارج خلوية للبكتيريا البحرية K. flava (F57) ذات خصائص مضادة للبكتيريا المرضية، وقد أسهمت في تعزيز فعّالية المضاد الحيوي Ciprofloxacin.
Received 16/10/2023
Revised 02/02/2024
Accepted 04/02/2024
Published Online First 20/08/2024
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