Evaluation of Hemolysis Activity of Zerumbone on RBCs and Brine Shrimp Toxicity

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Shaymaa Fadhel Abbas Albaayit
Rukesh Maharjan
Mariam Khan

Abstract

Zerumbone is a well-known compound having anti-cancer, anti-ulcer, anti-inflammatory and anti-hyperglycemic effects. During its use for the disease treatment, the membrane of erythrocyte can be affected by consumption of this bioactive compound. The current study was the first report of investigation of the hemolytic activities on human erythrocytes and cytotoxic profile of zerumbone. The toxicity of zerumbone on human erythrocytes was determined by in vitro hemolytic assay. Brine shrimp lethality assay was used to evaluate the cytotoxic effect of zerumbone at concentrations 10, 100 and 1000 μg/mL. The human erythrocyte test showed no significant toxicity at low concentrations, whereas hemolytic effect was amplified up to 17.5 % at the highest concentration. The half lethal concentration (LC50) value of zerumbone against brine shrimp was less than 1000 µg /mL (LC50=190 µg/ml) showing the significant toxic nature of this compound. These results provide a baseline in terms of the toxicity of therapeutic formulations from this compound to membrane erythrocytes with a great attention to the highest concentrations, which paves promise for drug development.

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Evaluation of Hemolysis Activity of Zerumbone on RBCs and Brine Shrimp Toxicity. Baghdad Sci.J [Internet]. 2021 Mar. 10 [cited 2024 Mar. 28];18(1):0065. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5434
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How to Cite

1.
Evaluation of Hemolysis Activity of Zerumbone on RBCs and Brine Shrimp Toxicity. Baghdad Sci.J [Internet]. 2021 Mar. 10 [cited 2024 Mar. 28];18(1):0065. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5434

References

Albaayit SFA, Rasedee A, Abdullah N, Abba Y. Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities. Asian Pac J Trop Biomed. 2020;10 (5): 232–238.

Albaayit SFA, Al-Khafaji AS, Alnaimy HS. In vitro macrophage nitric oxide and interleukin-1 beta suppression by Moringa peregrina seed. Turk J Pharm Sci. 2019;16 (3): 362–365.

Al-Bahrani RM, Radif HM, Albaayit SFA. Evaluation of potent silver nanoparticles production from Agaricus bisporus against Helicobacter pylori. Pak J Agri Sci. 2020;57 (4): 1197–1201.

Albaayit SFA, Ozaslan M. Cytotoxic and Urease Inhibition Potential of Moringa peregrina Seed Ethanolic Extract. Int J Pharmacol. 2019;15 (1): 151–15.

Albaayit SFA, Maharjan R. Immunomodulation of Zerumbone via Decreasing the Production of Reactive Oxygen Species from Immune Cells. Pak J Biol Sci. 2018;21 (9): 475–479.

Albaayit SFA, Rasedee A, Abdullah N. Zerumbone-loaded nanostructured lipid carrier gel facilitates wound healing in rats. Rev Bras Farmacogn. 2020;(30):272–278.

Bucker A, Falcao-Bucker NC, Nunez CV, Pinheiro CC, Tadei WP. Evaluation of larvicidal activity and brine shrimp toxicity of rhizome extracts of Zingiber zerumbet (L.) Smith. Rev Soc Bras Med Tro. 2013;46 (3): 377–380.

Sam MF, Hamid A, Ghazali AR, Louis SR, Budin SB. Protective Effects of Zingiber zerumbet Ethyl Acetate Extract on Hydrogen Peroxide-Induced Damage of Red Blood Cells. Sains Malays. 2019;48 (4): 781–790.

Ghramh HA, Khan KA, Ibrahim EH. Biological Activities of Euphorbia peplus Leaves Ethanolic Extract and the Extract Fabricated Gold Nanoparticles (AuNPs). Molecules. 2019;24 (7):1431.

Independent Ethics Committee of ICCBS, University of Karachi with informed consent ICCBS/IEC-028-HB-2017/PROTOCOL/1.0.

Raza A, Muhammad F, de Sousa DP, Khaliq T, Aslam B, Andrade L, Bashir S, Anwar MI, Shahid M, Qamar M. In vitro and in vivo toxicological evaluations of methyl ferulate, methyl p-coumarate, and pulegone 1, 2-epoxide. Pharm biol. 2016;54 (3): 523–529.

Ad’hiah AH, Ibraheem RM, Abbood KW. Modulation of cytokine production from cultured mononuclear cells of leukemia patients by Hypericum triquetrifolium Turra methanolic extract. Arab J Basic Appl Sci. 2018;25 (1): 13–9.

Albaayit SFA, Abba Y, Abdullah R, Abdullah N. Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers. Drug des devel ther. 2016;(10):1973.

Albaayit SFA, Abba Y, Abdullah R, Abdullah N. Effect of Clausena excavata Burm. f.(Rutaceae) leaf extract on wound healing and antioxidant activity in rats. Drug des devel ther. 2015;(9):3507.

Albaayit SFA, Abba Y, Abdullah R, Abdullah N. Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract. Evid Based Complement Altern. 2014;975450.

Al-naddawi THS, Albaayit SFA, khalaf ZZ. The Synergism and Antagonism behavior of Aqueous Extraction for Black Tea, Green Tea and Coffee against the Effectiveness of Certain Antibiotics. eurasia proc sci technol eng math. 2019;6 (6): 39–42.

Al-Ani LKT, Yonus MI, Mahdii BA, Omer MA, Taher JK, Albaayit SFA, Al-Khoja SB. First record of use Fusarium proliferatum fungi in direct treatment to control the adult of wheat flour Tribolium confusum, as well as, use the entomopathogenic fungi Beauveria. Ecol environ conserv. 2018; 24 (3): 29–34.

Haque MA, Jantan I, Arshad L, Bukhari SNA. Exploring the immunomodulatory and anticancer properties of zerumbone. Food Funct. 2017;8 (10): 3410–3431.

Zohra M, Fawzia A. Hemolytic activity of different herbal extracts used in Algeria. Int J Pharm Sci Res. 2014;5 (8): 495–500.

Mahomoodally F, Mesaik A, Choudhary MI, Subratty AH, Gurib-Fakim A. In vitro modulation of oxidative burst via release of reactive oxygen species from immune cells by extracts of selected tropical medicinal herbs and food plants. Asian Pac J Trop Med. 2012;5 (6): 440–447.

Amin K, Dannenfelser RM. In vitro hemolysis: guidance for the pharmaceutical scientist. J pharm sci. 2006;95 (6):1173–1176.

Mushtaq S, Abbasi BH, Uzair B, Abbasi R. Natural products as reservoirs of novel therapeutic agents. EXCLI J. 2018; 17: 420–451.

Riaz A, Rasul A, Hussain G, Zahoor MK, Jabeen F, Subhani Z, et al. Astragalin: a bioactive phytochemical with potential therapeutic activities. Adv Pharmacol Pharm Sci. 2018; 9794625.

Bucker A, Falcao-Bucker NC, Nunez CV, Pinheiro CC, Tadei WP.Evaluation of larvicidal activity and brine shrimp toxicity of rhizome extracts of Zingiber zerumbet (L.) Smith. Rev Soc Bras Med Tro. 2013;46 (3): 377–380.