Abstract
A simple, precise, rapid, and accurate reversed – phase high performance liquid chromatographic method has been developed for the determination of guaifenesin in pure from pharmaceutical formulations.andindustrial effluent. Chromatography was carried out on supelco L7 reversed- phase column (25cm × 4.6mm), 5 microns, using a mixture of methanol –acetonitrile-water: (80: 10 :10 v/v/v) as a mobile phase at a flow rate of 1.0 ml.min-1. Detection was performed at 254nm at ambient temperature. The retention time for guaifenesin was found 2.4 minutes. The calibration curve was linear (r= 0.9998) over a concentration range from 0.08 to 0.8mg/ml. Limit of detection (LOD) and limit of quantification ( LOQ) were found 6µg/ml and 18µg/ml respectively. The method was validated for its linearity, precision and accuracy .The proposed method was successfully applied for the determination of guaifenesin in syrups and industrial effluent samples.
Article Type
Article
How to Cite this Article
Ahmed, Nief Rahman and Lottfi, suhaib N.
(2013)
"High performance liquid chromatographic method for the determination of guaifenesin in pharmaceutical syrups and in environmental samples,"
Baghdad Science Journal: Vol. 10:
Iss.
3, Article 47.
DOI: https://doi.org/10.21123/bsj.2013.10.3.1014-1022
