Abstract
Flow-injection (FI) spectrophotometric method has been developed for the analysis of thymol in pharmaceutical preparations. The method is based on organic coupling reaction between thymol and 4-amino antipyrine in the presence of alkaline medium to form an intense stable red color complex with copper nitrate that has a maximum absorption at 490 nm. Optimum conditions for determination of the drug was investigated .The calibration graph was linear over the range of 5-500 µg.ml-1 of thymol . The limit of detection (LOD) and limit of quantification (LOQ) were 1.81 µg mL-1 and 3.60 µg mL-1 respectively .The proposed method was applied satisfactorily to the determination of thymol in mouth wash preparations. The procedure is characterized by its simplicity, accuracy and precision.
Keywords
Thymol, Flow-injection Spectrophotometric determination, 4-Aminoantipyrine, copper nitrate
Article Type
Article
How to Cite this Article
Al-Abachi, Mouayed Q. and Al-Najjar, Nada Ali
(2015)
"Flow Injection Spectrophotometric Determination of Thymol using 4-Aminoantipyrine and Copper(II) Nitrate,"
Baghdad Science Journal: Vol. 12:
Iss.
2, Article 13.
DOI: https://doi.org/10.21123/bsj.2015.12.2.332-339
