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Abstract

Inthis study 100 samples were collected from infected children with acute and chronic tonsillitis who attended to Al-Yarmook Teaching Hospital (ENT consultation clinic) from 5/12/2013 to1/3/2014. The result of laboratory culture was positive in 67 samples. Depending on their cultural, morphological and biochemical characterization of bacterial isolate of them were identified as (37.31%) belonged to Streptococcus pyogenesand the diagnosis is confirmed by the use of Remel Rapid STR System,(34.32%) belonged to S.parasanguinis, (11.94%) S.mitis, (11.94%) S.oralisand (4.47%) S.thoraltensis.Results confirmed that cup assay gave highest inhibition zone after 24 hrs compare with well diffusion methods for suspension of L.acidophilusgave highest inhibition zone after 48 hrs for incubation, while ahigh inhibition zone revealed for suspension of L.fermentumafter 24 hrs incubation. the study included also the measurement of the inhibition activity for bacteriocins produced by L.acidophilusbacteria against pathogenic bacteria on nutrient agar by well diffusion method in which results revealed stability of the bacteriocins produced towards PH which kept its activity with PH 4-6 for 24hrs, and the highest stability was with PH 4, however it lost a lot of its activity with acidic PH less than 2 and alkaline PH as8. The treatment of bacteriocins with salts such as Nacl it revealedlittle effect in inhibition zone within1 & 2% concetrations. The salt MgSo4& Kclshowed reduction in the inhibitory activity in thelow concentration, however thehigher concentration of salt caused great reduction and 5% concentration led to loss of inhibitory activity for bacteriocins completel.

Keywords

Tonsillitis, inhibition activity, S. pyogenes, L.acidophilus.

Article Type

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