Molecular Identification of Fusobacterium Isolates and limitation of Biofilm Formation Adhesion Gene (fadA) in Dental Outpatients

Authors

Aws Ibrahim Sulaiman, Department of Biology, College of Science, University of Mosul, Mosul, Iraq.Follow

Abstract

Fusobacterium are compulsory anaerobic gram-negative bacteria, long thin with pointed ends, it causes several illnesses to humans like pocket lesion gingivitis and periodontal disease; therefore our study is constructed on molecular identification and detection of the fadA gene which is responsible for bacterial biofilm formation. In this study, 10.2% Fusobacterium spp. were isolated from pocket lesion gingivitis. The isolates underwent identification depending on several tests under anaerobic conditions and biochemical reactions. All isolates were sensitive to Imipenem (IPM10) 42.7mm/disk, Ciprofloxacin (CIP10) 27.2mm/disk and Erythromycin (E15) 25mm/disk, respectively. 100% of Fusobacterium spp. isolates had 16S rDNA gene (360bp.), whereas two isolates had fadA gene (232bp.)

Keywords

Biofilm formation, FadA, Fusobacterium spp., 16S rDNA

Article Type

Article

How to Cite this Article

Sulaiman, Aws Ibrahim (2019) "Molecular Identification of Fusobacterium Isolates and limitation of Biofilm Formation Adhesion Gene (fadA) in Dental Outpatients," Baghdad Science Journal: Vol. 16: Iss. 4, Article 6.
DOI: https://doi.org/10.21123/bsj.2019.16.4.0843