Abstract
Spectrophotometric method was developed for the determination of copper(II) ion. Synthesized (2,2[O-Tolidine-4,4-bis azo]bis[4,5-diphenyl imidazole]) (MBBAI) was used as chromogenic reagent at pH=5. Various factors affecting complex formation, such as, pH effect, reagent concentration, time effect and temperature effect, have been considered and studied. Under optimum conditions concentration ranged from (5.00-80.00) µg/mL of copper(II) obeyed Beer`s Low. Maximum absorption of the complex was 409nm with molar absorpitivity 0.127x104 L mol-1 cm-1. Limit of detection(LOD) and Limit of quantification were 1.924 and 6.42 μg/mL, respectively. The stoichiometric composition of the chelate is 1:2 (Cu:MBBAI). Experimental results for studying some selected ions as interference were reported. The developed method was successfully applied to determine copper (II) ion in dental filling.
Keywords
Azo dye(MBBAI), Copper (II), Dental filling and Spectrophotometry.
Article Type
Supplemental Issue
How to Cite this Article
Hatam, Raghad Saad; Shaheed, ihsan Mahdi; and Hussain, Alaa Frak
(2020)
"Spectrophotometric Determination of Copper(II) using 2,2[O-Tolidine-4,4-bis azo]bis[4,5-diphenyl imidazole](MBBAI),"
Baghdad Science Journal: Vol. 17:
Iss.
1, Article 37.
DOI: https://doi.org/10.21123/bsj.2020.17.1(Suppl.).0287
