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Qais Ahmed Ibraheem, College of Agricultural Engineering Sciences, Basic Sciences, University of Baghdad, Baghdad, IraqFollow
Luma Hasan Alwan Al Obaidy, Department of Biology, College of Science for Women, University of Baghdad, Baghdad, Iraq*Corresponding author:lumaha_bio@csw.uobaghdad.edu.iq*ORCID ID:0000-00027649-45163Dean of High Institute for Infertility Diagnosis and Assisted Reproductive Techniques, Al Nahrain University, Baghdad, IraqE-mail:dr.manaltaha@ieri.nahrainuniv.edu.iqORCID ID:0000-0001-8722-3159Received 25/12/2019, Accepted 16/6/2020, Published8/9/2020This work is licensed under aCreative Commons Attribution 4.0 International License.Abstract:Polycystic syndrome (PCOS) is a considerable infertility disorder in adolescents and adult women in reproductive age. Obesity is a vigorous risk factor related to POCS. This study aims to evaluate the association of obesity and PCOS by investigating several parameters including: anthropological, biochemical (lipid profile, fasting blood sugar, glucose tolerance test, and hormonelevels (LH, FSH, LH/FSH ratio, Estradiol2 and Testosterone),and genetic parameters (Fat mass and Obesity associated gene (FTO) polymorphism at rs17817449) in 63 obese and non-obese PCOS women. The biochemical tests were investigated by colorimetric methods while FTO gene polymorphism was detected by PCR–RFLP. Lipid profile, FBS, GTT,hormones (LH, LH/FSHratio) in obese PCOS patients were significantly higher than non-obese non PCOS patients. It was found that the FTO variant TT risk genotype is a predisposing factor to obesity but not for PCOS. The study substantiated a possible familial risk factor for developing obesity among women in the same family.Key words: FTO, Obesity, PCOS, Polymorphism, rs17817449.Introduction:Polycystic ovary syndrome (PCOS) is a complex infertility disorder in adolescents (1) and adult women in the reproductive age (2). Stein and Leventhalwho were firstly linked between PCOS and obesity in 1935 when 3/7 of first PCOS diagnosed patients were obese (3). The main features of this syndrome are oligo/anovulation, and disturbance in androgen hormones leading to different ranges of hirsutism, weight gain, acne, and amenorrhea according to Rotterdam criteria (4,5). Overweight or being obese with bodymass index (BMI) greater than 30 kg/m2is a vigorous risk factor leading to PCOS women in around the age of 30 (5, 6, 7), in addition to represent a risk factor to cardiovascular disease, diabetes mellitus type 2, hypertension and several types of cancers(8). Obesity and PCOS are multifactorial diseases resulting from the interaction of genetics, metabolic, hormones, patient's life style, eating habits and psychological behavior(9). The potential genetic investigations focused on the expression ,mutationsand polymorphism in metabolism related genes, action related genes and genes that encode for reproductive controlling hormones and their receptor(10). Structural chromosomal aberration such as X chromosome aberration leading to abnormal follicular behavior and deletion of the loge arm of chromosomes 2, 11, 16, 19 were reported in some PCOS cases (11, 12, 13, 14 and 15). It is not clear whether obesity leads to PCOS or vice versa. The cornerstone for both PCOS and obesity is insulin resistance that leads toexcess levels of insulin and glucose uptake failure by adipose and muscle cells, increasing the risk ofFollow
Gulboy Abdolmageed Nasir, College of Agricultural Engineering Sciences, Basic Sciences, University of Baghdad, Baghdad, IraqFollow
Manal Taha Meteab Al-Obaidi, Dean of High Institute for Infertility Diagnosis and Assisted Reproductive Techniques, Al Nahrain University, Baghdad, IraqFollow

Abstract

Polycystic syndrome (PCOS) is a considerable infertility disorder in adolescents and adult women in reproductive age. Obesity is a vigorous risk factor related to POCS. This study aims to evaluate the association of obesity and PCOS by investigating several parameters including: anthropological, biochemical (lipid profile, fasting blood sugar, glucose tolerance test, and hormone levels (LH, FSH, LH/FSH ratio, Estradiol2 and Testosterone),and genetic parameters (Fat mass and Obesity associated gene (FTO) polymorphism at rs17817449) in 63 obese and non-obese PCOS women. The biochemical tests were investigated by colorimetric methods while FTO gene polymorphism was detected by PCR–RFLP. Lipid profile, FBS, GTT, hormones (LH, LH/FSH ratio) in obese PCOS patients were significantly higher than non-obese non PCOS patients. It was found that the FTO variant TT risk genotype is a predisposing factor to obesity but not for PCOS. The study substantiated a possible familial risk factor for developing obesity among women in the same family.

Keywords

FTO, Obesity, PCOS, Polymorphism, rs17817449

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