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Abstract

This study aimsto investigate the possible role of circulating microRNA-142-3p (miR-142-3p) in the development of graves disease (GD) and its association withtheantibody directed againstthyroid stimulating hormone receptor (TSHR-Ab) production in patients with GD. Forty patients with positive TSHR-Ab enrolled in this study were divided,based on treatment,into (22 untreated (newly diagnosed) and 18 treated patients)and based on family history(30 with positive family history and 10 with negative family history). In addition to forty healthy subjectswith sex and age matching asacontrol group. The expression level of circulating miR-142-3p was determined by two steps reverse transcription polymerase chain reaction (RT-PCR) technique. Results showthat there is a significant elevation(p < 0.01) in the expression of miR-142-3pintheserum of both treated and untreated patients compared with controlsand in patients with positive family history compared with negative family history. While its expression is non-significantly lower (P > 0.05) in the serum of treated patients comparedwith untreatedones. It has beenfound that miR-142-3p expression was positively correlated with levelsof TSHR-Ab,FT3,and FT4.Inaddition, the miR-142-3pexpressionhas a good diagnostic accuracy with sensitivity (82%) and specificity (80%). In conclusion, the differential expression of miR-142-3p between patients and healthy controls appearsas a potential biomarker for diagnosis of GD and thepositive correlationof miR-142-3p with TSHR-Absuggesting the contribution ofthis miRNA in the development of GD.

Keywords

Circulating microRNAs, MicroRNA-142-3p, Graves disease, Thyroid stimulating hormone receptor antibody, Reverse transcription polymerase chain reaction

Article Type

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