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Abstract

This study aims to create an easy method for simultaneously measuring nimesulide and flurbiprofen in pharmaceutical formulations. This research provides insight into how variations in buffer concentration, pH levels, and acetonitrile content influence the separation mechanism of two non-steroidal anti-inflammatory drugs (NSAIDs). The retention time of two NSAIDs was increased with an increase of eluent pH value from 3 to 5.5. When acetonitrile content increased from 5 % to 50 %, the retention of two target drugs decreased, indicating hydrophobic and ionic interactions. The RP - HPLC system with UV detection accomplished separation (250×4.60 mm, 130Å , and 5) using a C8 Hyper Clone BDS column. The acetonitrile and acetate buffer mixture is used as the mobile phase gradient elution at a detection wavelength of 254 nm and a 1 mL/min flow rate. The linear ranges were 0.05-12.50 and 0.03-17.35 μg.ml-1 for nimesulide and flurbiprofen, respectively. LOD 0.030, 0. 020μg.ml-1 and LOQ 0.091, 0.060μg.ml-1 for nimesulide and flurbiprofen, respectively. The verification findings demonstrate the suitability of the proposed methods for quantifying NSAIDs in pharmaceutical formulations.

Keywords

Flurbiprofen, Nimesulide, NSAIDs drugs, Pharmaceutical formulations, RP-HPLC

Subject Area

Chemistry

Article Type

Article

First Page

1116

Last Page

1123

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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