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Abstract

Gastrointestinal microbial infections have increased especially between children and represent a community burden. The present experiment was designed to examine the combined impact of local wheat bran bioactive compounds and Bacteriocin over locally isolated pathogens including S. aureus, E. coli and C. albicans and evaluate their antimicrobial and antibiofilm eligibility. Local wheat Ibaa99 bran extract consists of phenolic compounds, phytic acid and inulin, Bacteriocin was purified from Lacticaseibacillus paracasei that was identified as a new strain (namely MOIQ35) by the 16S rRNA gene using the PCR technique and after its registration with The National Center for Biotechnology Information (NCBI). The combined antimicrobial activity was examined by well diffusion assay and MIC method at concentrations 1 mg/ml for wheat bran extract and 0.54 mg/ml for bacteriocin. Meanwhile, antibiofilm dispersal considerations have been completed by the microtiter plate method. The outcomes revealed that the highest inhibition observed over
S. aureus isolates ranged from 21.5 ± 0.1 mm to 40 ± 0.1 mm, and the highest inhibition observed over E. coli isolates ranged from 18 ± 0.1 mm to 38 ± 0.1 mm, and 38.5 ± 0.1 mm against C. albicans. Moreover, the biofilm suppression tests showed a high reduction in biofilm against C. albicans isolate with OD (0.11). In conclusion, the extract of Ibaa99 wheat bran in combination with Bacteriocin has synergistic antibacterial and antifungal effects and is a biofilm repressor.

Keywords

Antibacterial, Antifungal, Bacteriocin, Biofilm, Wheat bran extract

Subject Area

Biology

Article Type

Article

First Page

1549

Last Page

1559

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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