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Abstract

This study aimed to determine the antibacterial effect of combined acetylsalicylic acid (ASA) and azithromycin (AZM) against resistant Salmonella enterica. For this, the MIC and MBC of both drugs were determined, and a checkerboard test, a time-killing assay, and scanning electron microscopy were performed. The results showed that ASA had an antibacterial effect with MIC value of 16000 μg/ml and MBC value of 32000 μg/ml. Also, the combination of ASA with AZM has an additive effect with FICI interaction value 1.5 at 8000 μg/ml of ASA and 16 μg/ml of AZM, and this has a direct effect on preventing further development of resistance in S. enterica exposed for 21 days. The time-killing results showed that it had a concentration-dependent effect, and the combination of the two drugs gave a better result in reducing the number of bacteria at 8000 μg/ml+16 μg/ml for ASA and AZM, respectively with P ≤ 0.05. SEM showed concentration-dependent morphological changes, which extended from membrane rupture to loss of cell contents and death. Also, the resistance induction assay was able to keep the resistance of AZM at a lower concentration of 16 μg/ml when combined with ASA. Finally, we conclude that ASA has an antibacterial effect in vitro, and their combination is useful in achieving better therapeutic effects in infections and preventing the development of resistance.

Keywords

Checkerboard test, Interaction, MIC, Scanning electron microscopy, Time-killing assay

Subject Area

Biology

Article Type

Article

First Page

2254

Last Page

2263

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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