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Abstract

Curtobacterium flaccumfaciens SHGH was associated with Koch's postulates about wilt disease in Olea europaea (olive trees) located in Mosul, Iraq. Since olive trees are an important cash crop for Iraq, a whole-genome analysis of SHGH was conducted. The complete genome was sequenced using the IIIumina MiSeq sequencer and uploaded to GenBank under the accession number JAUMSN000000000.1. The circular chromosome consists of 3,834,306 bp, 70.7 percent GC content, 4,176 protein-coding sequences, 53 RNA genes, 7 ribosomal RNA genes, and 42 transfer RNA genes. According to the in silico DNA-DNA hybridization technique are 11 strains closely related to the SHGH strain. The 16S rRNA gene sequence analysis showed that the SHGH strain has 100% similarity with C. flaccumfaciens LMG 3645 and 99% similarity with the Curtobacterium allii 20TX0166 gene over 100 replicates in the bootstrap test. The SHGH strain analysis showed the presence of many genes encoding pathogenicity-associated enzymes such as pectate lyase, glycosyl hydrolase, serine proteinase, beta-1,4-glucanase, and 1,4-beta-xylanase enzymes. Furthermore, the genome analysis of the SHGH strain showed that it contained peptidases, glycosidases, and potential glycopolymer-degrading domains in prophage-derived regions. These help the microbe establish a biofilm and colonize with other microbes to form a microbial community, which is a necessary step for the progression of many bacterial plant diseases, such as bacterial wilt disease.

Keywords

Biofilm formation, Curtobacterium flaccumfaciens, Olea europaea, Virulence associated genes, Whole genome sequencing

Subject Area

Biology

Article Type

Article

First Page

2264

Last Page

2272

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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