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Abstract

Cancer is a multistage defect and very complex disease. It is distinguished by particular genetic mutations that lead to a loss of management of cellular functions during uncontrolled growth of normal cells leading to malignant cells. CA18 occurs by the abnormal growth of cells in colon. SPP1 is multifunctional protein. The aim of this study is to study genetic polymorphism of SPP1gene in CA18 patients along with healthy individuals to find the relationship with CA18 and compare both globally. DNA was extracted and the genetic polymorphism of the SPP1gene was determined. Genetic analysis of SPP1 (in rs11730582 T/C region) polymorphism for the two groups showed of (CC) 1 (6.7%) in healthy control and 6 (20.0%) in patients. The results of (TC) showed 8 (53.3%) in healthy control and 14 (46.7%) in patients. The result of (TT) was 6 (40.0%) in healthy control and 10 (33.3%) in patients, it indicates no significance between the two groups in this region at (p>0.05). The results showed SPP1 (in rs11439060 GG/G- region) for the two groups 2 (13.3%) dilation/dilation in healthy control and 14 (46.7%) in patients, while they gave 8 (53.3%) insertion/dilation in healthy control and 12 (40.0%) insertion/dilation in patients. A result of insertion/insertion was 5 (33.3%) in healthy control, while 4 (13.3%) in patients. These results mean that there was no significance between two groups in this region at (p>0.05). The result of rs11730582 SNP of SPP1 gene was close to the results of East Asian.

Keywords

CA18 Colon Cancer, CC homozygous genotype, PCR Polymerase Chain Reaction, SPP1 Osteopontin, TC heterozygous genotype

Subject Area

Chemistry

Article Type

Article

First Page

2581

Last Page

2586

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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