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Abstract

The hydrophilic interaction liquid chromatography (HILIC) conjugated with UV spectrometry was employed to the determination of two antihistaminic drugs (Acrivastine and fexofenadine) in pharmaceutical formulations using two lab-made zwitterionic HILIC stationary phases (ZIC-2 and ZIC-5). The study aimed to investigate the impact of separation parameters including acetonitrile (ACN) content, pH of the eluent, and acetate buffer concentration on the retention behavior of Acrivastine and fexofenadine. A mixture of 3.0 mM acetate buffer (pH 4.75) and ACN (30:70) was used as eluent, with a flow rate of 0.6 mL/min. The detection wavelength was controlled at 267nm. The linearity of Acrivastine and fexofenadine is 0.09-13.5 and 0.09-9 µg/mL on the ZIC-2 and ZIC-5 columns, respectively. The proposed methods exhibited a high level of precision (RSD ≤ 0.82), these methods determined the LOD for Acrivastine in ZIC-2 and ZIC-5 to be (0.030 – 0.008 µg/ml) and LOQ (0.090 – 0.024 µg/ml), respectively. LOD values for fexofenadine were determined to be (0.050 – 0.020 µg/ml) for ZIC2 and ZIC5, respectively. Additionally, LOQ values were found to be (0.1515 – 0.060 µg/ml) for ZIC2 and ZIC5, respectively.

Keywords

Acrivastine, Antihistaminic drugs, Fexofenadine, Pharmaceutical Preparations, and ZIC-HILIC.

Subject Area

Chemistry

Article Type

Article

First Page

2517

Last Page

2526

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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