•  
  •  
 

Abstract

Plants can synthesize a large number of different active compounds and ingredients, which can be influenced under stress conditions. The plant in vitro culture technique is considered a suitable and useful method to propagate and protect the medicinal and herbal plants, and to extract active natural compounds. The main target of this work was to obtain callus of Calendula officinalis explants stimulated by Nano zinc, and then to extract the natural glycosides from callus in vitro culture and apply them against certain bacterial and fungal isolates. The explant of C. officinalis was grown on media containing a mix of hormones, at concentrations of 0.5 mg·L-1 of benzylaminopurine (BA) and 1-naphtaleneacetic acid (NAA). The dry callus was extracted with methanol, and the extracted glycoside were hydrolysed to release the aglycone. Six selected species of bacteria were isolated from wound infections, identified and verified using the Vitek2 technique. Three species of yeasts were used to investigate the influence of oleanolic acid (OA) on the viable counts of colonies. Current work revealed that Proteus mirabilis and Candida krusei were isolated the most affected by OA. The application of elicitors, such as nano zinc, can be considered as an useful elicitation technique to enhance OA biosynthesis and accumulation in callus culture, and it seems to exert a promising effect against certain bacteria and yeasts.

Keywords

Antimicrobial activity, Calendula officinalis, Callus, Nano zinc, Oleanolic acid

Subject Area

Biology

Article Type

Article

First Page

924

Last Page

931

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Download

Share

 
COinS