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Abstract

This study presents the development of electrochemical biosensors for the glycated albumin (GA) sensitive detection as a crucial biomarker for monitoring glycaemic control in diabetes mellitus (DM). A ferrocene-labelled DNA aptamer specific to glycated albumin was developed as an electrochemical aptamer-based sensor to detect GA. The amine-functionalized graphene nanoplatelets (GNPs), with their enhanced surface area, provided an optimal substrate for aptamer immobilization through covalent interactions. The layer-by-layer full EDC/NHS-GNPs-Aptamer biosensor, tested by cyclic voltammetry (CV), exhibited strong redox peaks due to the good electron transfer activity between the biosensing structures. The GA-aptamer binding was studied using square wave voltammetry (SWV), and the data revealed a linear relationship for a wide range of concentrations between 10 and 10,000 μ g/mL with a low limit of detection (LOD) of 0.12 μg/mL. The performance of EDC/NHS-GNPs-Aptamer-GA displayed high selectivity and good response to the low concentrations, making it a valuable tool for clinical diagnostics and the management of diabetic patients.

Keywords

Biosensor, DNA aptamer, Glycated albumin, Graphene nanoplatelets, Diabetes mellitus

Subject Area

Physics

Article Type

Article

First Page

964

Last Page

975

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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