Quantitative Analysis of Thiamine, Riboflavin, and Niacin Vitamins by High-Performance Liquid Chromatography in Pharmaceutical Sample Tablets and Drops

Authors

Sarra A. Abrahem, Department of Chemistry, College of Science, Al-Nahrain University, IraqFollow
Suhad A. Ibrahim, Department of Chemistry, College of Science, Al-Nahrain University, IraqFollow
Alaa Sh. Abdulbari, Department of Applied Chemistry, College of Applied Sciences, University of Technology- Iraq, Baghdad, IraqFollow
Noor M. Ali, Department of Chemistry, College of Science, Al-Nahrain University, IraqFollow
Maryam Y. Hashim, Industrial Property and Technical Design Directorate, the Central Organization for Standardization and Quality Control (COSQC), Baghdad, IraqFollow

Abstract

Levering cutting-edge high-performance liquid chromatography techniques with a C-18 column, we meticulously crafted a gradient of MeOH and glacial CH₃COOH (96–5%), pulsating through at a mesmerizing 1.2 ml/min. With ultraviolet detection at a precise wavelength of 245 nm, we unraveled the mysteries of these vital nutrients. Thiamine reveals itself at a retention time of 3.5 min, followed by Riboflavin at 8.1 min and Niacin at 10.8 min. The findings of this study were not just a spectacle of scientific powers but were also rigorously benchmarked against commercial drugs, ensuring accuracy and reliability in our analysis.

Keywords

High-performance liquid chromatography, Niacin, Riboflavin, Thiamine

Subject Area

Chemistry

Article Type

Article

First Page

818

Last Page

824

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite this Article

Abrahem, Sarra A.; Ibrahim, Suhad A.; Abdulbari, Alaa Sh.; Ali, Noor M.; and Hashim, Maryam Y. (2026) "Quantitative Analysis of Thiamine, Riboflavin, and Niacin Vitamins by High-Performance Liquid Chromatography in Pharmaceutical Sample Tablets and Drops," Baghdad Science Journal: Vol. 23: Iss. 3, Article 5.
DOI: https://doi.org/10.21123/2411-7986.5240