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Abstract

An effective method for the in vitro propagation of Tulipa gesneriana was developed using Murashige and Skoog (MS) medium. Seeds were first cultured on hormone-free MS medium, achieving %90 germination after 30 days. Seedlings were obtained, and flowering stem explants excised from these seedlings were used for callus induction. The best callus formation was obtained on MS medium supplemented with thidiazuron (TDZ1.5mg/L) and 2,4-dichlorophenoxyacetic acid (2,4-D1.5mg/L). Shoot proliferation was most effective on MS medium containing 2.0 mg/L benzyladenine (BA) and 0.2 mg/L indole-3-butyric acid (IBA), and bulb formation was promoted by 0.5 mg/L BA, producing 3–4 bulbs per explant, while root induction was achieved with 1.0 mg/L naphthalene acetic acid (NAA), generating up to 5 roots per plantlet. Plantlets with well-developed shoots and roots exhibited survival rates ranging from 70-90% during acclimatization in peat moss and peat moss: soil (1:1) substrates. This study provides a reliable method for large-scale tulip propagation and conservation.

Keywords

Bulb formation, Callus induction, Shoot regeneration, Thidiazuron, Tulip

Subject Area

Biology

Article Type

Article

First Page

1558

Last Page

1566

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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