Different Chitinase Activity in Nutrient-Enriched Multi-Antagonistic Streptomyces sp. and Trichoderma sp. against Root-Knot Nematodes Meloidogyne sp.

Authors

• Tri Mujoko, Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Jawa Timur, 60294 Surabaya IndonesiaFollow
• Penta Suryaminarsih, Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Jawa Timur, 60294 Surabaya IndonesiaFollow
• Safira Rizka Lestari, Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Jawa Timur, 60294 Surabaya IndonesiaFollow

Abstract

The threat of root-knot nematodes to horticultural and plantation crops is a grave concern. To combat this issue, using bacteria and fungi as biological control agents is an effective and efficient approach. This research aims to evaluate the efficacy of Streptomyces sp. strains PS23104 and Trichoderma sp. when grown on nutrient-enriched media as a biological control agent for root-knot nematodes Streptomyces sp. PS23104 and Trichoderma sp. were cultured on enriched EKG media with malt extract, glucose nitrate, pure coconut water, and molasses, while potato extract media without nutrient addition served as the control. The findings indicate that both Streptomyces sp. PS23104 and Trichoderma sp. exhibited optimal performance on potato extract media with 10 ppm molasses, virgin coconut water, malt extract, and glucose nitrate. These five treatments were then utilized in the antagonism test, where 150 J2 Meloidogyne sp. in 4 mL of suspension were transferred into a 5 cm petri dish. 1 mL of each suspension of Streptomyces sp. PS23104 and Trichoderma sp. were added to the petri dish, along with the treatment groups (potato extract media as control, potato extract media plus 10 ppm malt extract, potato extract media plus 10 ppm glucose nitrate, potato extract media plus 10 ppm pure coconut water, and potato extract media plus 10 ppm molasses). The mortality rate of Meloidogyne sp. in each treatment was 82.78% in molasses, 75.5% in pure coconut water, 65.25% in malt extract, 65.30% in glucose nitrate, and 53.25% in the control. The same treatment was also utilized to measure the chitinase enzyme activity produced by Streptomyces sp. and Trichoderma sp. The chitinase enzyme activity in the treatment groups ranged from 6–15 U ml-1, while in the control group, it was 3 U ml-1. This research demonstrates that the addition of nutrients to the growth medium of Streptomyces sp. PS23104 and Trichoderma sp. can enhance their antagonistic activity against Meloidogyne sp. by augmenting the chitinase activity produced by these microorganisms.

Keywords

Chitinase, Enriching media, Meloidogyne sp., Streptomyces sp. PS23104, Trichoderma sp.

Subject Area

Biology

Article Type

Article

First Page

2380

Last Page

2391

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite this Article

Mujoko, Tri; Suryaminarsih, Penta; and Lestari, Safira Rizka (2026) "Different Chitinase Activity in Nutrient-Enriched Multi-Antagonistic Streptomyces sp. and Trichoderma sp. against Root-Knot Nematodes Meloidogyne sp.," Baghdad Science Journal: Vol. 23: Iss. 7, Article 1.
DOI: https://doi.org/10.21123/2411-7986.5346