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Abstract

Multidrug-resistant (MDR) bacteria are becoming one of the most pressing problems worldwide and are becoming difficult to treat; therefore, nanotechnology is a promising alternative technology. The current study aimed to biosynthesize chitosan Saccharomyces cerevisiae nanoparticles (ScNPs) and evaluate their antibacterial activity against hypervirulent Klebsiella pneumoniae (HvKp). Five K. pneumoniae isolates from different clinical sources were screened for extended-spectrum beta-lactamase (ESBL) production and virulence tests. ScNPs were synthesized and characterized. The antibacterial activity of ScE and ScNPs was determined. The result showed that five bacterial isolates were confirmed as K. pneumoniae and displayed highly virulent and resistant capabilities, hence classifying them as hypervirulent K. pneumoniae isolates. The result of the ScNPs syntheses revealed the occurrence of chitosan Tripolyphosphate (TPP) as a crosslinking agent; the process was visually established by color changes and alterations in solubility. The characterization results of ScNPs showed new absorbance peaks with crystalline patterns with sizes ranging from 26 to 48 nm and distinct aggregates and rough surfaces. The antibacterial activities results showed that ScNPs exhibited significant antibacterial activity against clinical isolates of HvKp, due to the synergistic effects of chitosan membrane disruptive characteristics as a proved by total phenolic compounds (TPC) and total flavonoid compounds (TFC), the result reveled both ScE and ScNPs have with value (197,93.5) and (124.5,78.3) for TPC and TFC respectively that hence their antioxidants and antimicrobial activity against different antibiotic resistant pathogenic microorganisms, this findings provided evidenced to employing nanoparticles as a substitute for antibiotics.

Keywords

Antibiotic-resistant bacteria, Bioactivity, Chitosan, Microencapsulation technology, Nanoparticles, Saccharomyces cerevisiae, Yeast

Subject Area

Biology

Article Type

Article

First Page

2425

Last Page

2440

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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