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Abstract

The current study was conducted to compare and evaluate the increasing or decreasing levels of LaDXS1 ``1-deoxy-D-xylulose 5-phosphate synthase'' gene expression of four treatments of callus initiate on Murashige and Skoog medium (MS) with various application of 2,4-dichlorophenoxyacetic acid (2,4-D) and Benzyl adenine (BA) from leaves of Lavandula Angustifolia consider one of species used for oil extraction through total RNA extraction from callus for the four treatments then reverse-transcribed to complementary DNA (cDNA) then amplified by RT-qPCR technique as a relative quantitation method .The results demonstrated that gene expression of LaDXS1 was varied between treatments. The highest gene expression of LaDXS1 in the second treatment was recorded at 2.282 gene folding as compared with control group which record 1 gene fold, while the lowest treatment is 1.580 copy number of gene. Our conclusion is that LaDXS1 gene expression elevated by using very economical and available hormonal combination has been affected to increase lavender oil production in callus.

Keywords

Callus, Gene expression, LaDXS1, Lavandula angustifolia, qRT-PCR

Subject Area

Biology

Article Type

Article

First Page

2722

Last Page

2728

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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