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Abstract

Ulcerative colitis (UC) is an idiopathic inflammatory disorder of the colon and the most prevalent form of inflammatory bowel disease (IBD) worldwide. Cytotoxic T cells are one of the most important cells in the immunopathogenesis of IBD. These cells produce serine protease granzymes that initiate an apoptotic cascade that may contribute to intestinal tissue damage in IBD. Therefore, this study aimed to evaluate the tissue mRNA expression and serum levels of Granzyme A and Granzyme B in UC patients and to investigate their association with the proinflammatory cytokines IL-1β and Oncostatin M. A total of 80 Iraqi individuals were enrolled in this study, comprising 40 UC patients and 40 controls. Then, informed consent was obtained from all participants. Tissue mRNA expression of Granzyme A and Granzyme B was assessed by RT-qPCR, while serum levels of Granzyme A, Granzyme B, IL-1β , and Oncostatin M were measured using ELISA. The results revealed that tissue mRNA expression levels of Granzyme A and Granzyme B were significantly elevated in both inflamed and uninflamed colonic tissues of UC patients compared with the control group. Serum levels of Granzyme B, IL-1β , and Oncostatin M were also significantly increased in UC patients, whereas Granzyme A showed no significant difference. Moreover, a significant positive correlation was observed between tissue granzyme expression and proinflammatory cytokines. In conclusion, these findings suggest that these markers may serve as potential biomarkers of intestinal inflammation and may have prognostic value. They also indicate a potential role for T-cell-mediated cytotoxic effector mechanisms in UC.

Keywords

Granzyme A, Granzyme B, IL-1ß, Oncostatin-M, Ulcerative colitis

Subject Area

Biology

Article Type

Article

First Page

3098

Last Page

3107

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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