Abstract
Base analogue, 5-Bromouracil used to study the response of G-system isolates G3( Bacillius spp)G12 (Arthrobacter spp)& G21 (Brevibacterium spp)which belong to different genera in comparison to the effect of standard mutagen Nitrosoquanidine (NTG), when applied at similar conditions with graudal concentrations. Results showed that the analogue affected the survival fraction after treatment for 15 mins. The mutagenic effect of BU was lagged for that of NTG upon using streptomycin and rifampicin resistance as genetic markers, although the former was more indicative. BU recorded less effeciency (mutant / Ig of mutagen) than that of comparative control (NTG). Isolate G27 showed higher mutability by BU than by NTG. Using the latter G12 showed the highest mutability.
Article Type
Article
How to Cite this Article
Al-Khafaji, Zahra M. and Al-Azawi, Ghaith L.
(2006)
"Developing of Bacterial Mutagenic Assay System for Detection of Environmental and Food Mutagens
III-Using Mutagenesis with Base Analogue
5-Bromouracil,"
Baghdad Science Journal: Vol. 3:
Iss.
1, Article 3.
DOI: https://doi.org/10.21123/bsj.2006.671
