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Abstract

Watermelon seeds showed also a higher production even more than the legumes. Measured enzyme activity for watermelon milled seeds was found to have 353 unit/gm seeds. However, stabilization characters were studied which showed that there is a very high stability under extraction conditions and under different storage parameters. Results also showed that the enzyme stability decreased gradually in the presence of 2-mercaptoethanol, ethylene diamine tetra acetate di sodium (EDTA) at (1 mmolar) for each concentration. Addition of glycerol (10%) to a buffer's solution used is the process of enzyme activity. Enzyme from C. vulgaris was purified through different steps as extraction, dialysis, heat treatment, acetone fractionation, ionic exchange as a (Batch wise) employing ion exchange column using diethyl amino ethyl cellulose (DEAE cellulose),. Separation results showed that the enzyme found to have two shapes (A & B) and it can be easil separatedfrom each other, using NaCl (0.2 and 0.3) Molar subsequently. The specific activity were measured and found to be (141.66) and (58.57 unit/mg protein) since the activity of enzyme A is superies that of enzymes by two folds. Purification steps for enzyme A were completed through filtration on the column of Sephadex G-200 and the specific activity for this purified enzyme reached up to 204.54 unit/mg protein.

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