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Abstract

3 cm long shoots of MM101 and Amara apple rootstocks from proliferating cultures were placed for rooting on a growth regulators - free Ms medium with full, half, one third, quarter and one fifth strength of it's ionic concentration, also cultures incubating condition at 25 +2°C (under full light 1000 lux for 16 hr/day, Full ligh + covering of rooting zone with black tape and continuos dark) were studied. Results showed that both rootstocks diffed in their response to all treatments. MM106 was superior to Amara with the rooting percentage (100%) especially, when their shoots were cultured on one fifth and one third Ms salts and keeping in the light with or without covering the rooting zone with black tape. While shoots of Amara rootstock gave percentage 20% on one fifth and one fourth MS salts. However the rootstock MM106 were significantly superior in number and lengths of the roots with the leaf number and higher plant lengh when incubated condittion at full light and full light covering of rooting which reached to 2.13 root/shoot,3.42 cm.8.8 leaf/plant and 7.8 cm, respectively, and 1.46 root /shoot,4.58cm 6.4 leaf/ plant and 6.62cm, respectively, under full light +covering of rooting. Whereas no rooting was achieved when shoots of both rootstocks were incubated in continuous darkness for 8 weeks. Reduction of salt concentrations resulted in enhancement of rooting percentage, as well as number and length of roots for rooted shoots. Among the tested salt concentrations the most suitable for rooting was one third MS salts wich gave the higest number and length of roots per shoot (2.01 roots and 4.31cm) respectively, whereas at one fifth MS salts a higher plant length and leaf number (6.57 cm and 7.87 leaf) respectively were obtained.For both rootstocks no roots were obtained with full or half MS salt concentration medium.

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