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Abstract

Three medicinal plants were collected from their natural habitat. The volatile oil was extracted by steam distillation procedure To detect the quality and the quantity of each extracted oil, Gas chromatography and HPLC technique have been used. The effectiveness of each of essential oil was tested by using agar-well diffusion method. The extracted oil from Cymbopogon citratus showed high inhibition for S.aureus by 40mm diameter of inhibitory zone with minimum inhibition concentration (MBC) 3.125%. The Thymbra spicata oil showed 39.67mm diameter of inhibition zone with MBC 25% for the same group of the S.aureus bacteria The extracted oil of Mentha longifolia showed 33.67mm diameter inhibition with MBC 12.5% The mixture of two groups of the extacted oil showed high degree of inhibition for the isolated sample of bacteria. The extract of C.citratus gave higher inhibitory effect when mixed with T.spicata extract against S.aureus. The mixture of three extracted oils showed a different effect from that when used singly. These extracted oils hadn t shown any inhibitory effect against tested Gram negative bacteria.

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