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Abstract

Among twenty one species and isolates of fungi, the production of fibrinolytic protease in fifteen of them was recorded. The maximum lysis times of human plasma clot were 40,41,44,45,46,46,54 and 64 min recorded by Aspergillus niger (AB-11), Pleurotus ostreatus (white variety), Pleurotus ostreatus (gray variety), Aspergillus oryzae (AB-21). Pleurotus sajor-caju.Trichoderma harzianum, Coprinus cinerus and Agaricus bitorquis, respectively, when these fungi grown on the liquid medium No.1 composed essentially of sucrose.soya bean powder and yeast extract powder in a shacked culture conditions. The positive fungi for human plasma clot lysis test were evaluated for their fibrinolytic activity in various types of liquid and solid media. The maximum human plasma clot lysis time were 38, 43 and 44 min recorded by Aspergillus niger (AB-11). Aspergillus oryzae (AB 21) and Trichoderma harzianum, respectively, when these fungi grown on the liquid medium No.6 composed essentially of glycerin, Mg SO4, KH2PO4, CaCl2 and (NH4)2SO4. The results showed that all tested fungi produced a fibrinolytic protease when grown on a solid medium composed of Sus wastes (produced from extracted medicinal plant, Glycyrrhiza glabra) and the maximum human plasma clot lysis time were 37, 39 and 39 min recorded by white and gray variety of Pleurotus ostreatus and Trichoderma harzianum respectively.

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