Abstract
Two groups of chronic hepatitis B and C virus patients were divided into Pre-treated patients (25 CHB patients with positive HBs Ag for more than 6 months and 40 CHC patients), and post-treated patients [12 CHB patients (4, 6, and 2 were treated with lamivudine, IFN-a and combination of LMV+ IFN-a respectively), and 27 patients for CHC (3, 13 and 11 patients were treated with Ribavirin, IFN-a and combination therapy (RBV+ IFN-a) respectively] These patients were followed up for 6 months. By using ELISA technique, levels of IL-6, IL-10, IFN-y and TNF-a were measured in vivo and in vitro (supernatant of PBMCs stimulated with PHA) and compared with healthy control. The mean level of IL-6, IL-10 and TNF-a in CHB patients showed significant differences (P<0.05) between pre- and post-treated patients in vivo and in vitro, while there was no significant difference in IFN- y between pre- and post-treated patients in vivo and in vitro. The difference between control and CHB patients was highly significant (P<0.0001) in IL-6, IL-10 and TNF-a levels in vivo and in vitro. In CHC patients the mean levels of IL-6, IL-10, IFN-y and TNF-a showed significant difference between pre- and post-treated patients in vivo and in vitro. There was highly significant difference (P<0.0001) between patients and control in IL-10 levels. Hence, these observations indicate the predominance of Th2 cytokine, which promote the persistency of the CHB and CHC virus
Article Type
Article
How to Cite this Article
Al-azzawi, Raghad Harbi; Mohammed, Ghanima Sadiq; and Al-khalidi, Nawal Mahdi
(2009)
"Estimation of cellular immune response by evaluation of some cytokines in immunopathogenesis of chronic hepatitis B and C pre- and post- treated Iraqi patients (in vivo and in vitro),"
Baghdad Science Journal: Vol. 6:
Iss.
3, Article 14.
DOI: https://doi.org/10.21123/bsj.2009.6.3.533-541