Abstract
Type-1 fimbriae have been extracted from E.coli which was previously isolated, the yield was 2.8 mg fimbriae/10 g bacteria , the crude fimbriae was partially purified by several solubilization and recrystilization methods using a series of buffer and solutions to decrease the impurities, the yield of fimbriae from all these steps was 2.1 mg fimbriae / 10 g bacteria. Adhesive protein FimH was extracted from fimbriae by separation then partially purified by Gel-chromatography sephadex G-100, the yield of FimH was 250 µg/ml, while fimbriae protein was 50.6 µg/ml. The partially purified FimH revealed one clear band in SDS-PAGE, as well as protein fimbriae with molecular weight (28300,17500) Dalton respectively, when compared with standard protein curve.
Article Type
Article
How to Cite this Article
M, Alice K. and alaubydi, Mouruj A.
(2010)
"Extraction and partial purification for adhesive protein FimH from type-1 pili which isolated from uorpathogenic E.coli,"
Baghdad Science Journal: Vol. 7:
Iss.
1, Article 85.
DOI: https://doi.org/10.21123/bsj.2010.11911
