Abstract
This paper present a simple and sensitive method for the determination of DL-Histidine using FIA-Chemiluminometric measurement resulted from oxidation of luminol molecule by hydrogen peroxide in alkaline medium in the presence of DL-Histidine. Using 70μl. sample linear plot with a coefficient of determination 95.79% for (5-60) mmol.L-1 while for a quadratic relation C.O.D = 96.44% for (5-80) mmol.L-1 and found that guadratic plot in more representative. Limit of detection was 31.93 μg DL-Histidine (S/N = 3), repeatability of measurement was less that 5% (n=6). Positive and negative ion interferances was removed by using minicolume containing ion exchange resin located after injection valve position.
Article Type
Article
How to Cite this Article
Shakir, Issam M.A. and Turkey, Nagam S.
(2010)
"New Method for the Determination of DL-Histidine by FIA and Chemiluminometric Detection,"
Baghdad Science Journal: Vol. 7:
Iss.
2, Article 10.
DOI: https://doi.org/10.21123/bsj.2010.7.2.927-938
