Simultaneous spectrophotometric method for determination of both ciprofloxacin and cephalexin by using H-point standard addition method
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Abstract
An easy, simple and accurate method for estimating ciprofloxacin in the presence of cephalexin or vice versa in a mixture of the two. It has been possible to successfully apply the proposed method H-point standard addition method (H-PSAM) in the estimation of ciprofloxacin in the presence of cephalexin interfering at wavelengths 240-272.3 nm and with different concentrations of ciprofloxacin 4-18 μg.ml-1. As well as the estimation of the cephalexin in the presence of ciprofloxacin interfering at wavelengths 262-285.7 nm and with different concentrations of cephalexin 6-18 μg.ml-1 in a mixture of them. The results showed the absence of any interferences by the additives that the drugs may contain on these compounds and within the detection limits of ciprofloxacin equal to 0.1732 μg.ml-1 and drug cephalexin equal to 0.4620 μg.ml-1. RSD% less than 2%. The method has been successfully applied for the determination of the two drugs in some pharmaceutical preparations. The proposed method is considered one of the low-cost methods and it does not need to introduce the properties in a series of reactions and fixation of the reaction conditions to estimate them. Rather, it is done by estimating the property after dissolving it directly in distilled water in the presence of another drug with it in a mixture. It is considered one of the successful methods in the assessment, especially for properties that are close in spectrum absorption for which it is not possible to find ways to separate the mixture more accurately than this proposed method without overlapping and the effects of one on the other. The proposed method in this research was successful in the estimation of both drugs ciprofloxacin and cefalexin in a mixture without drug interaction with the other.
Received 06/01/2023
Revised 02/04/2023
Accepted 10/04/2023
Published Online First 20/09/2023
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Berrin G A, Sabriye P, Esengül K, Gizem Y B. Determination of ciprofloxacin in pharmaceutical dosage, human serum and urine, using molecularly imprinted polymer modified electrode by voltammetry. Eur J Sci Technol. 2020; 20 (S): 859-865. https://doi.org/10.31590/ejosat.796654
Lionel S A, Livia M B, Tercio P O, Elfrides E S S. LC method for quantitative determination of Ciprofloxacin in ophthalmic ointment. Drug Anal Res. 2021; 5(2): 25-29. https://doi.org/10.22456/2527-2616.118365
Ejele A E, Ukiwe L N, Enenebeaku C K and Ejele N U. Evaluation of Drug Quality (III): Determination of Ciprofloxacin Hydrochloride Concentration in Pharmaceutical Tablets. Niger J Chem Res. 2015; 20: 19-28. https://doi.org/10.1186/s13104-017-2507-y
Ramzia E, Asmaa A E, Ehab E, Asmaa A M. Simultaneous determination of ciprofloxacin hydrochloride and metronidazole in spiked human plasma by ultra-performance liquid chromatography-tandem mass spectroscopy. J Appl Pharm Sci. 2016; 6 (3): 41-47. https://doi.org/10.7324/JAPS.2016.60307
Teshome G, Bisratewongel T, Feleke Z, Bhagwan S C. Salting-out assisted liquid–liquid extraction for the determination of ciprofloxacin residues in water samples by high performance liquid chromatography–diode array detector. BMC Chem. 2019; 13(28): 1-10. https://doi.org/10.1186/s13065-019-0543-5
Shrinivas S, Revanasiddappa M. Analytical stability indicative method development and validation by high pressure liquid chromatography for assay in ciprofloxacin hydrochloride drug substances. Am J Analyt Chem. 2015; 6: 719-730. https://doi.org/10.4236/ajac.2015.69069
Madhavi K, Chamundeswari K, Prasanthi C. Development and validation of RP-HPLC method for simultaneous estimation of ciprofloxacin and fluocinolone acetnnide in bulk and pharmaceutical dosage form. Int J App Pharm. 2020; 12(1): 134-138. https://doi.org/10.22159/ijap.2020v12i1.35492
Syed N R, Muhammad A, Irfana M, Islam U K, Syed S R, Ghulam M. Stability indicating RP-HPLC method for simultaneous determination of ciprofloxacin and dexamethasone in binary combination. J. Chil. Chem. 2017; 62(3). https://doi.org/10.4067/s0717-97072017000303572
Abdussamed M A S. Indirect spectrofluorimetric method for the determination of cefotaxime sodium, ciprofloxacin hydrochloride and famotidine in pharmaceuticals using bromate-bromide and acriflavine dye. Baghdad Sci. J. 2020; 17 (1): 66-77. https://doi.org/10.21123/bsj.2020.17.1.0066
Palled M S, Hadaki D, Mahendra K C, Sunil S J, Shailendra S S. Method development and validation of ciprofloxacin HCl and ornidazole by UFLC in combined dosage form. Ind. J Pharm Edu Res. 2019; 53 (3): S373- S379. https://doi.org/10.5530/ijper.53.3s.108
Livia U, Elena D, Vladimir V, Oxana V, Veaceslav G, Sergiu P. Development and validation of an assay method for ciprofloxacin hydrochloride determination in combination ear drops. Chem J Moldova. ASM.MD. 2019; 14(2): 56-61. https://doi.org/10.19261/cjm.2019.607
Kawther A S. Spectrophotometric determination of ciprofloxacin by ion pair complex formation with bromothymol blue. J Coll Bs Edu. 2019; 17(72): 93-100. https://doi.org/10.35950/cbej.v17i72.4501
Maha A M, Sumayha M A, Jasim M S. Derivative Spectrophotometric determination for simultaneous estimation of isoniazid and ciprofloxacin in mixture and pharmaceutical formulations. Metheds Obj Chem Anal. 2020; 15 (3): 105-110. https://doi.org/10.17721/moca.2020.105-110
Muna A K, Maha A M. Simultaneous determination of ciprofloxacin hydrochloride and mebeverine hydrochloride by derivative spectrophotometry. Ibn Al-Haitham J Pure Appl Sci. 2014; 27(3): 448-461. https://jih.uobaghdad.edu.iq
Abdul Majeed K A, Imad T H, Seemaa H A. Determination of the ciprofloxacin hydrochloride drug in some pharmaceuticals using manufactured membrane selective electrodes. Sys Rev Pharm. 2020; 11(6): 622- 626. https://doi.org/10.31838/srp.2020.6.92
Amina M A. Synthesis new liquid selective electrodes of ciprofloxacin hydrochloride for determination ciprofloxacin in pure form and pharmaceuticals preparation. Baghdad Sci. J. 2017; 14(4): 787-792. https://doi.org/10.21123/bsj.2017.14.4.0787
Abbas S F, Mohammed Z T, Asmaa M A, Saadiyah A D. Development of an ecological-friendly method for ciprofloxacin determination and cloud point extraction in pharmaceuticals using Fe (II) (FeSO4.7H2O). IOP Conf. Series: Mater Sci Eng 2020; 871 (1): 1-8. https://doi.org/10.1088/1757-899X/871/1/012028
Izabella K, Krystian P, Rafał G, Paweł K. Simultaneous determination of ciprofloxacin and ofloxacin in animal tissues with the use of capillary electrophoresis with transient pseudo-isotachophoresis. Molecules. 2021; 26 (6931): 1-10. https://doi.org/10.1590/S0103-5053
Izabella K, Krystian P, Paweł K. Simple, fast and reliable CE method for simultaneous determination of ciprofloxacin and ofloxacin in human urine. Sci Rep. 2022; 12(7729): 1-9. https://doi.org/10.1038/s41598-022-11747-y
Mykola Ye B, Yuliia Yu S. Comparison of three independent methods of cephalexin determination by means of potassium caroate. Ars Pharm. 2017; 58(2): 59-65. https://doi.org/10.4321/S2340-98942017000200003
Neda I M. Simple two spectrophotometric methods for estimation of cephalexin in pure and pharmaceutical dosage form. Samarra J. Pure and Appl. Sci. 2021; 3 (1): 37-45. https://doi.org/10.54153/sjpas. 2021.v3i1.166
Mohamed Th A, Elham S S. Indirect Spectrophotometric determination of cefalexin monohydrate, ceftriaxone sodium and cefotaxim sodium in pharmaceuticals using N-bromosuccinimde and evans blue dye. J Educ Sci. 2020; 29 (3): 11-31. https://doi.org/10.33899/edusj.2019.125959.1009
Ravin J, Mrudul K. Spectrophotometric determination of some antibiotics using bromophenol blue as ion pair reagent. Pharm Anal Acta. 2015; 6 (6): 1-6. https://doi.org/10.4172/2153-2435.1000380
Maha A M, Elham N M, Kawther A S. Simultaneous determination of cephalexin and cefixime by first and second derivative ultraviolet Spectrophotometry. J Coll Bs Edu. 2016; 22 (93): 89-100. https://doi.org/10.35950/cbej.v22i93.7852
Sabriye P, Besnik U, Gizem Y. Voltammetric Analysis of Cephalexin and Cefazolin in Pharmaceutical Formulations and Biological Samples. J. Turk. Chem. Soc. 2019; 6 (2): 217-224. https://doi.org/10.18596/jotcsa.469028
Narsu K K, Rajiya Sd, Bhulakshmi A, Pavan K D, Gayathri D K, Prudhvi C P, Kalyani V. Analytical method development and validation of cephalexin by RP-HPLC method. J Emerg Technol Innov Res. 2019; 6 (6): 696-699. https://doi.org/10.13140/RG.2.2.11276.74882
Manal M H, Ahmed M S, Tariq K I. RP – HPLC developed analytical method for cephalexin determination in pure and pharmaceutical preparations. College of Education for Pure Science, University of Diyala. 2020; 2(2): 1-8. https://doi.org/13140/RG.2.2.11276.74882
Sabrina G C, Rafaela Z, Ytan A S, Bruna C, Daniel K, Peterson H, Ani C W, Daiane H, Eduardo M E, Clarice S, Lucélia H. Degradation of micropollutant cephalexin by ultraviolet (UV) and assessment of residual antimicrobial activity of transformation products. Water Sci Tech. 2021; 84(2): 374- 383. https://doi.org/10.2166/wst.2021.170
Huda G S, Dalia M J, Amina M A, Ahmed A. Synthesis liquid membrane electrodes of cephalexin hydrochloride. Sysrevpharm. 2020; 11(6): 710 -716. https://doi.org/10.31838/srp.2020.6.105
Asmaa G D, Nagham N H, Lazeeza S O. H-point standard addition method for the simultaneous spectrophotometric determination of captopril and hydrochlorothiazide in pharmaceutical formulations. Jordan J Chem. 2021; 16(2): 77-85. https://doi.org/10.47014/16.2.4
Muhammed W S, Diyar S A. H- point standard addition method for simultaneous determination of phenylephrine hydrochloride, chlorpheniramine maleate, and paracetamol as a ternary mixture in pharmaceutical formulations. J. Indian Chem. Soc. 2022; 99 (7): 100526. https://doi.org/10.1016/j.jics.2022.100526
Sara A K, Fadam M A. Spectrophotometric method for the simultaneous determination of pyridoxine hydrochloride & phenylephrine hydrochloride via H-point standard addition method. AIP Conf Proc. 2022; 2398 (030019). https://doi.org/10.1063/5.0094172
Theia’ A N, Andrel A B, Hassan Y A. H-Point standard addition method (HPSAM) in simultaneous spectrophotometric determination of binary mixtures: an overview. App Spec Rev. 2011; 46(6): 607-623. https://doi.org/10.1080/05704928.2011.570836