Amplification of D18S51, D8S1179, and FES loci of DNA Samples Isolated from Guitar String Stored at Room Temperature
DOI:
https://doi.org/10.21123/bsj.2024.10206Keywords:
D18S51, D8S1179, FES, STR Loci, Guitar String, CrimeAbstract
The act of criminality is an unlawful behavior that constitutes a criminal offense. Criminals use various ways to hide evidence at the crime scene. Forensic experts often find DNA on items found at the crime scene, one of the types of evidence is a guitar. Guitar is one of the musical instruments often played by humans that can be used as a comparison. Methods: This study used 18 guitar string samples that had been used for five minutes and incubated at room temperature. The 18 samples were then divided into three groups, in which each group consisted of six samples and incubated for one, five, and 10 days. DNA identification was then carried out using a UV spectrometer for DNA quantification and the DNAzol method for DNA extraction. Results: The mean result of DNA quantification on day 1 was 152.57 ± 48.02 ng/μL, day 5 was 138.66 ± 47.66 ng/μL, and day 10 was 87.09 ± 9.07 ng/μL. Polymerase chain reaction was carried out using three STR primers loci, namely D18S51 (290 - 366 bp), D8S1179 (203 - 224 bp), and FES (222 - 250 bp), followed by visualization using the silver nitrate method. The final results showed that all samples could be amplified using the D18S51, D8S1179, and FES STR loci.
Received 14/11/2023
Revised 07/07/2024
Accepted 09/07/2024
Published Online First 20/11/2024
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Copyright (c) 2024 Abdul Hadi Furqoni, Anindita Riesti Retno Arimurti, Meri Meri, Ni Luh Ayu Megasari, Indah Nuraini Masjkur, Qurrota A’yunil Huda, Intan Sari Nuraini, Luluk Hermawati
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