Studying the effect of thymol-containing Nano emulsion extracted from thyme on angiogenesis controlling genes VEGF and VEGFR in a human liver cancer cell line
DOI:
https://doi.org/10.21123/bsj.2024.10591Keywords:
Hepatocellular carcinoma, Nanoemulsion, Thymol, Tumors, VEGF, VEGFR.Abstract
Thyme oil contains thymol (2-isopropyl-5-methylphenol), a naturally occurring, colorless crystalline phenolic compound with a characteristic odor. It is considered an antioxidant. Thymol has low solubility in water and good solubility in oil. Thymol nanoemulsion, dissolved in sesame oil, was prepared by converting it into nanodroplets. From a biological perspective, biological surfaces can effectively adsorb nanoemulsion droplets for more efficient biological activities. Nanoemulsions are spherical particles that act as a carrier for drug molecules, and range in size from 10-1000 nanometers. Because of the anticancer effect of thymol, it can be used as a suitable treatment to prevent angiogenesis in hepatocellular carcinoma. performed a cytotoxicity investigation using the MTT assay, in which a human hepatocellular carcinoma cell line was treated with different concentrations of thymol nanoemulsion. The results indicated that as the concentration increased, cell viability decreased. RNA was then extracted and C-DNA was synthesized. The expression levels of VEGFR and VEGF genes in nanothymol- and free-thymol-treated cells and in untreated cells were then examined. The results showed that the expression of this gene in cells treated with free thymol decreased by 5.33 times compared to untreated cells, but when the cells were treated with nanothymol, the level of gene expression decreased by about 10.02 times.
Received 03/01/2024
Revised 09/07/2024
Accepted 11/07/2024
Published Online First 20/11/2024
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