The Histological Effect of Aeromonashydrophila on liver of male albino mice

Abstract

        Two hundred of stool samples  from different age groups(less than 5 to 40 years) were collected from Al-Elwea , Al-Kendy , Children welfare and Baghdad Teaching hospital from September 2009 to May 2010 . Using the isolation methods & diagnostic laboratory tests, Aeromonashydrophila was isolated according to the growth on MacConkey , blood &TCBS agar . The sensitivity test for diagnostic antibiotic O129 was done , also by biochemical test using api20E  . The percentage of isolation for Aeromonashydrophila was 5% for all patients . The hemolysin assay was also done to select the proper isolate for  the study . The isolate 2 showed high activity  for hemolysin at its 640 unit/ ml by using human blood . The LD50 was detected  and found it was 10¹⁰ bacterium / mouse when orally administrated  in this study using 28 male of albino mice age 6-8 weeks and weight 20-25 g were divided into 4 group (7 mice / groups and were orally administrated with 0.2 ml of inoculums 10⁴ , 10⁶ , 10⁸ bacterium / mouse) for 10 days .   These results show pathological changes in liver after 10 days . The changes  include congestion ,hemorrhage , degeneration & necrosis . Tha bacteria Aeromonashydrophila had a wide spread & can infect all age groups & it had a negative effect on liver