Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System Wisam H. Salo

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Baghdad Science Journal

Abstract

Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-?-galactosidase Control Vector which encoded ?-galactosidase, maximum transfection efficiency were achieved with TransFast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA/ml, this is indicate that TransFast™ Reagent can be used as an efficient transfection agent to deliver foreign DNA into human larynx carcinoma cell line Hep-2 and expression of the transgene efficiently.

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1.
Journal BS. Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System: Wisam H. Salo. Baghdad Sci.J [Internet]. 2011Sep.4 [cited 2021Aug.2];8(3):728-35. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1284
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